We narrowed to 4,189 results for: Pet2

1. MZB220

   Plasmid

   #221771

   Purpose

   Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arl1 (ARL1 Human)
   

   Use

   Tags

   CPD

   Expression

   Bacterial

   Mutation

   Q71L

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MZB222

   Plasmid

   #221772

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   ArfGEF1 (ARFGEF1 Human)
   

   Use

   Tags

   10xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-28a-YTH domain of YTHDC2

   Plasmid

   #228779

   Purpose

   Protein expression plasmid for YTH domain of YTHDC2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDC2 (YTHDC2 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TTR C10S S85C G83R

   Plasmid

   #184747

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, G83R

   Promoter

   T7

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TTR C10S S85C Y78F

   Plasmid

   #184746

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, Y78F

   Promoter

   T7

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TTR C10S S85C A81T

   Plasmid

   #184745

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, A81T

   Promoter

   T7

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TTR C10S S85C K80E

   Plasmid

   #184744

   Purpose

   bacterial expression of huamn TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, K80E

   Promoter

   T7

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SNAP-Kindlin

   Plasmid

   #186136

   Purpose

   For expression of recombinant Snap-labeled kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TTR C10S S85C I84S

   Plasmid

   #184748

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, I84S

   Promoter

   T7

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNC2

    Plasmid

    #72438

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg

    Promoter

    T7 and n/a

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK516

    Plasmid

    #71578

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to glutamine

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK524

    Plasmid

    #71580

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes asparagine-347 to alanine

    Promoter

    T7

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK513

    Plasmid

    #71576

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-294 to alanine

    Promoter

    T7

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK564

    Plasmid

    #71581

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes serine-71 to alanine

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK517

    Plasmid

    #71579

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to aspartate

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK514

    Plasmid

    #71577

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamine-435 to alanine

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK512

    Plasmid

    #71575

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes arginine-228 to glutamate

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-MRPL15

    Plasmid

    #31345

    Depositor

    Linda Spremulli

    Article

    Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

    Insert

    mitochondrial ribosomal protein L15 mature form (MRPL15 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Has the amino acids in the predicted mature form …

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pJK048

    Plasmid

    #75230

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and n/a

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge