We narrowed to 29,111 results for: ple

1. pCAG-OSF-VPS34

   Plasmid

   #99327

   Purpose

   Component for mammalian expression of recombinant PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

   Insert

   VPS34 (PIK3C3 Human)
   

   Use

   Tags

   Strep-Strep-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

   Plasmid

   #156463

   Purpose

   Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

   Depositor

   Andre Stiel

   Article

   Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

   Inserts

   Heme Oxygenase
   ReBphP-PCM
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. As0

   Plasmid

   #123158

   Purpose

   Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, ParsR-ABS67, PhrpLE, PrinA

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. "AND-gate" receiver plasmid

   Plasmid

   #127676

   Purpose

   Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

   Insert

   LuxR, GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 for LuxR, pLux for GFP

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-MBP-NRBF2

   Plasmid

   #99330

   Purpose

   Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

   Insert

   Nuclear receptor-binding factor 2 (NRBF2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. RS35075_pMQ30_KO_Kan

   Plasmid

   #185390

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS35075 upstream flanking homology region
   RR42_RS35075 downstream flanking homology region
   nptII kanamycin resistance marker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY095

   Plasmid

   #84744

   Purpose

   Expresses huLbCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   GFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pS44i8GM

   Plasmid

   #207527

   Purpose

   Conditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
   

   Use

   CRISPR; Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC153-dual-dCas9VP96-sgExpression

    Plasmid

    #48239

    Purpose

    Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP96

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK284 (Stag-3FLAG-Hygro)

    Plasmid

    #72800

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Hygro
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p21 shRNA2

    Plasmid

    #25869

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Insert

    p21 (Cdkn1a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

    Plasmid

    #138419

    Purpose

    CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BioTAP-tag

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ10713

    Plasmid

    #183958

    Purpose

    U6-crSox2-CAG-hyperdCas12a-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    crRNA targeting Sox2
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK283 (Stag-3FLAG-Neo)

    Plasmid

    #72799

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Neo
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

    Plasmid

    #217704

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGR6 (ADGRG6 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-840

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-MBP-TEVpCS-ADGRL2 CTF-FLAG

    Plasmid

    #217707

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL2 (ADGRL2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-824

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. GIT1A-FLAG

    Plasmid

    #15225

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNG1256

    Plasmid

    #149710

    Purpose

    Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    rpoZ (rpoZ )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGH10 - RlucFluc minus 1 HIV-1

    Plasmid

    #198224

    Purpose

    Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

    Depositor

    Isha Jain

    Article

    Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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