We narrowed to 3,399 results for: FRI;

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pBAD-tdTEVDB_C-15A/G4C

Plasmid

#177205

Purpose

Permuted td intron with evolved initiation sequence for the expression of circular mRNA

Depositor

Stephen Fried

Article

Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

Insert

Permuted GFP with evolved initiation sequence for circular mRNA

Tags

6x His

Expression

Bacterial

Mutation

Permuted sfGFP after residue 52 & Changed Cyt…

Promoter

araBAD

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1-GFP

Plasmid

#185573

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1L-GFP

Plasmid

#185574

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1L

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64-AGS-mCherry

Plasmid

#135596

Purpose

Expression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1

Plasmid

#185571

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1 (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpAGS-dGoLoco1-GFP

Plasmid

#185577

Purpose

for overexpression

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-dGoLoco1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-SpGalpha-i

Plasmid

#185568

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

Galpha-i

Use

In vitro transcription (mrna synthesis)

Tags

GFP, S-Tag

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAP35

Plasmid

#250142

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK049

Plasmid

#250135

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK050

Plasmid

#250136

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D2) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK011

Plasmid

#250137

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(IDR) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Mutation

K82R kinase-dead mutant

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRK084

Plasmid

#250141

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-3(GGGGS)-RGG) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP36

Plasmid

#250143

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP37

Plasmid

#250144

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D2-IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK047

Plasmid

#250145

Purpose

Expression of mutant PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(FL)(R123E, K126E, K129E) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Mutation

R123E, K126E, K129E

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK015

Plasmid

#250134

Purpose

Expression of full-length PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(FL) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK036

Plasmid

#250138

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK042

Plasmid

#250139

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-IDR) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK082

Plasmid

#250140

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-RGG) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1(118-1274)

Plasmid

#221207

Purpose

ScChd1-aa118-1274 chromatin remodeler

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[R126A/R130A]

Plasmid

#221197

Purpose

ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

R126A, R130A

Promoter

T7

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[Y137A]

Plasmid

#221198

Purpose

ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

Y137A

Promoter

T7

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[∆ChEx]

Plasmid

#221199

Purpose

ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa142-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-XlH3[ChEx]

Plasmid

#221200

Purpose

Xenopus laevis histone H3 with Nterminal tail swapped for ChEx domain of ScChd1.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3[ChEx]

Expression

Bacterial

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-Xl-H3∆Nterm[R42]

Plasmid

#221201

Purpose

Xenopus laevis histone H3 with N-terminal tail deleted.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3∆Nterm[R42]

Expression

Bacterial

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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Klotho (membrane)

Plasmid

#17712

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

V5

Expression

Mammalian

Mutation

P101S

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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Klotho (secreted)

Plasmid

#17713

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

His and V5

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pGEM-601[insert(+1) TA-rich]

Plasmid

#221196

Purpose

601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

601[insert(+1)TA-rich]

Use

Unspecified

Mutation

601 has an additional mutation added 22 bp from d…

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CohIII-CBM-HIS-ybbR

Plasmid

#60866

Purpose

Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

Depositor

Hermann Gaub

Article

Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

Inserts

CohIII
CBM

Tags

6xHIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

Cysteine 63 to Serine in CBM module

Promoter

T7

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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