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pWSK129-stm2585-gp130

Plasmid

#174404

Purpose

Bacterial expression of sarA-gp130 chimera under native promoter.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Use

Tags

Expression

Bacterial

Mutation

Promoter

Endogenous

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgBRSK1

Plasmid

#138705

Purpose

Expresses a human BRSK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgBRSK1 human (BRSK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgBRSK1

Plasmid

#138686

Purpose

Expresses a human BRSK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgBRSK1 human (BRSK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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TESK1 gRNA (BRDN0001149431)

Plasmid

#77890

Purpose

3rd generation lentiviral gRNA plasmid targeting human TESK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TESK1 (TESK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TESK1 gRNA (BRDN0001487046)

Plasmid

#77891

Purpose

3rd generation lentiviral gRNA plasmid targeting human TESK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TESK1 (TESK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TESK1 gRNA (BRDN0001145109)

Plasmid

#77892

Purpose

3rd generation lentiviral gRNA plasmid targeting human TESK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TESK1 (TESK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRSK1 gRNA (BRDN0001146674)

Plasmid

#77785

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRSK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRSK1 (BRSK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
BRSK1 gRNA (BRDN0001145478)

Plasmid

#77786

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRSK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRSK1 (BRSK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TSSK1B gRNA (BRDN0001148046)

Plasmid

#76671

Purpose

3rd generation lentiviral gRNA plasmid targeting human TSSK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TSSK1B (TSSK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TSSK1B gRNA (BRDN0001148592)

Plasmid

#76668

Purpose

3rd generation lentiviral gRNA plasmid targeting human TSSK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TSSK1B (TSSK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TSSK1B gRNA (BRDN0001147904)

Plasmid

#76669

Purpose

3rd generation lentiviral gRNA plasmid targeting human TSSK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TSSK1B (TSSK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TSSK1B gRNA (BRDN0001144733)

Plasmid

#76670

Purpose

3rd generation lentiviral gRNA plasmid targeting human TSSK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TSSK1B (TSSK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK1-beta

Plasmid

#197121

Purpose

Expression of MYC-tagged PSK1-beta in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

Insert

TAOK2 variant beta (aa1-1049) (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type variant beta

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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[pSK131] pCOLADuet p14|MP1

Plasmid

#136145

Purpose

bacterial expression of p14 and MP1

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

p14 and MP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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[pSK104] pET28 SLC38A9N-His

Plasmid

#136142

Purpose

bacterial expression of SLC38A9 N term fragment (1-119)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Use

Tags

L-IFP-F[1]

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
p663-UBC-miniTurbo-V5-TSSK1B_IDG-K

Plasmid

#170616

Purpose

Gateway destination clone of TSSK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-TSSK1B (TSSK1B Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-TESK1_IDG-K

Plasmid

#170614

Purpose

Gateway destination clone of TESK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-TESK1 (TESK1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-BRSK1_IDG-K

Plasmid

#135269

Purpose

Gateway destination clone of BRSK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-BRSK1 (BRSK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p667-UBC-BRSK1-V5-miniTurbo_IDG-K

Plasmid

#135258

Purpose

Gateway destination clone of BRSK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

BRSK1-V5-miniTurbo (BRSK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge