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Plasmid#116115PurposeLentiviral expression of ARAF S217FDepositorInsertARAF (ARAF Human)
UseLentiviralTagsExpressionMutationS217FPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ARAF-P216L
Plasmid#116113PurposeLentiviral expression of ARAF P216LDepositorInsertARAF (ARAF Human)
UseLentiviralTagsExpressionMutationP216LPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARAF gRNA (BRDN0001147899)
Plasmid#75660Purpose3rd generation lentiviral gRNA plasmid targeting human ARAFDepositorInsertARAF (ARAF Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARAF gRNA (BRDN0001148605)
Plasmid#75662Purpose3rd generation lentiviral gRNA plasmid targeting human ARAFDepositorInsertARAF (ARAF Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX459-puro-hARAF-1
Plasmid#185367PurposeFor mammalian expression of guide RNA: caccgTGGTCTACCGACTCATCAAG that targets human ARAFDepositorInsertARAF (ARAF Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ARAF-R188H
Plasmid#116114PurposeLentiviral expression of ARAF R188HDepositorInsertARAF (ARAF Human)
UseLentiviralTagsExpressionMutationR188HPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
R777-E010 Hs.ARAF-nostop
Plasmid#70294PurposeGateway ORF clone of human ARAF [NM_001256196.1] without stop codon (for C-terminal fusions)DepositorInsertARAF (ARAF Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-puro-hARAF-shRNA-1
Plasmid#185370PurposeFor tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAFDepositorInsertARAF (ARAF Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shABC2
Plasmid#186714PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shABC1
Plasmid#186713PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 FL (1-440)
Plasmid#210425PurposeBacterial expression of MBP-p62 FL (1-440) with 3C cleavage siteDepositorInsertp62 FL (NUP62 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPTM p62 PB1 (1-102)
Plasmid#210427PurposeBacterial expression of MBP-PB1(1-102) with TEV cleavage siteDepositorInsertp62 PB1 (1-102) (NUP62 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPTM p62 PB1 ZZ (1-167)
Plasmid#210429PurposeBacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage siteDepositorInsertp62 PB1 ZZ (1-167) (NUP62 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 dUBA (1-389)
Plasmid#210426PurposeBacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage siteDepositorInsertp62 FL dUBA (1-389) (NUP62 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPTM p62 PB1 (1-122)
Plasmid#210428PurposeBacterial expression of MBP-PB1(1-122) with TEV cleavage siteDepositorInsertp62 PB1 (1-122) (NUP62 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
NmMetNI-WT
Plasmid#172114PurposeExpression of MetN and MetI membrane proteinsDepositorInsertsMetN
MetI
UseTags10x His Tag and EnterokinaseExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ
Plasmid#172111PurposeExpression of Methionine Binding Protein (MetQ) with signal sequenceDepositorInsertMethionine Binding Protein
UseTags10x Histidine Tag and N.meningitidis signal seque…ExpressionBacterialMutationChanged Proline 22 to AlaninePromoterT7 PromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HRPT2
Plasmid#11048DepositorInsertHRPT2 (CDC73 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HRPT2 L64P
Plasmid#11052DepositorInsertHRPT2 L64P (CDC73 Human)
UseTagsFlagExpressionMammalianMutationL64PPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)
Plasmid#183254PurposeExpression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vectorDepositorInsertTissue factor (F3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only