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Addgene
Showing: 21 - 40 of 101 results

  1. PMmEos3.2

    Plasmid
    #203755
    Purpose
    Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    Lyn n-term (LYN Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203755-plasmid-map-sequence-id-403338

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  2. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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  3. mEos3.2FP

    Plasmid
    #203757
    Purpose
    Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    NRas c-term (NRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203757-plasmid-map-sequence-id-403337

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  4. mEos3.2GPI

    Plasmid
    #203759
    Purpose
    Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    CD58 c-term (CD58 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203759-plasmid-map-sequence-id-404788

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  5. mEos3.2-Lifeact-7

    Plasmid
    #54696
    Purpose
    Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mEos3.2-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    54696-plasmid-map-sequence-id-137746

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  6. pUCmEos3.2:cat

    Plasmid
    #78100
    Purpose
    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.
    Depositor
    Peng Chen
    Article
    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1
    Insert
    mEos3.2-cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  7. mEos3.2-Rad21-N

    Plasmid
    #66951
    Purpose
    Localization: Centromere, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Rad21 (RAD21 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. mEos3.2-Vinculin-N-21

    Plasmid
    #66952
    Purpose
    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    V234L in Vinculin
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. mEos3.2-MYR-N-5

    Plasmid
    #62769
    Purpose
    Localization: Cytoskeleton , N1 Cloning Vector, Excitation:507 / 572, Emission:516 / 580
    Depositor
    Michael Davidson
    Insert
    MYR
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. mEos3.2-Telethonin-C-18

    Plasmid
    #62770
    Purpose
    Localization: Scaffold, C1 Cloning Vector, Excitation:507 / 572, Emission:516 / 580
    Depositor
    Michael Davidson
    Insert
    Telethonin
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    62770-plasmid-map-sequence-id-199347

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  11. mEos3.2-Telethonin-N-18

    Plasmid
    #62771
    Purpose
    Localization: Scaffold, N1 Cloning Vector, Excitation:507 / 572, Emission:516 / 580
    Depositor
    Michael Davidson
    Insert
    Telethonin
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. mEos3.2-LaminA-C-18

    Plasmid
    #57463
    Purpose
    Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. mEos3.2-LaminB1-10

    Plasmid
    #57464
    Purpose
    Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. mEos3.2-LASP2-C-18

    Plasmid
    #57465
    Purpose
    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    LASP2 (NEBL Chicken)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57465-plasmid-map-sequence-id-210807

    Enlarge

  15. mEos3.2-LASP2-N-18

    Plasmid
    #57466
    Purpose
    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    LASP2
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57466-plasmid-map-sequence-id-213184

    Enlarge

  16. mEos3.2-MannII-N-10

    Plasmid
    #57467
    Purpose
    Localization: Mannosidase/Golgi, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MannII (Man2a1 Mouse)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    aa 1-112 of MannII
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57467-plasmid-map-sequence-id-213115

    Enlarge

  17. mEos3.2-MAPTau-N-10

    Plasmid
    #57468
    Purpose
    Localization: Microtubules, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MAPTau (MAPT Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57468-plasmid-map-sequence-id-202381

    Enlarge

  18. mEos3.2-MCPA2-C-18

    Plasmid
    #57469
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MCPA2 (Capza2 Mouse)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57469-plasmid-map-sequence-id-210751

    Enlarge

  19. mEos3.2-MCPA2-N-18

    Plasmid
    #57470
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MCPA2
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57470-plasmid-map-sequence-id-213182

    Enlarge

  20. mEos3.2-MCPB1-C-18

    Plasmid
    #57471
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MCPB1
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57471-plasmid-map-sequence-id-216903

    Enlarge

Showing: 21 - 40 of 101 results