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Plasmid#132413Purposeoverexpression in human cellsDepositorInsertmRuby3
UseTagsExpressionMammalianMutationnoPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3
Plasmid#107737PurposeIn the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.DepositorInsertsynaptophysin-GCaMP6s, mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDx_mRuby3-SYFP2
Plasmid#189758PurposeDual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysisDepositorInsertmRuby3-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn-FLEX.iGABASnFR.mRuby3
Plasmid#112169PurposeGABA SensorDepositorInsertiGABASnFR
UseAAVTagsmRuby3ExpressionMutationPromoterSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3
Plasmid#112170PurposeGABA SensorDepositorInsertiGABASnFR.F102G
UseAAVTagsmRuby3ExpressionMutationF102GPromoterSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3
Plasmid#107736PurposeIn the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.DepositorInsertsynaptophysin-GCaMP6s, mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoternEFAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/DdmRuby3
Plasmid#154305Purposecloned with the codon-optimized mRuby3 gene for expression in Dictyostelium discoideumDepositorInsertmRuby3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn-FLEX.iGABASnFR.F102Y.Y137L.mRuby3
Plasmid#112171PurposeGABA SensorDepositorInsertiGABASnFR.F102Y.Y137L
UseAAVTagsmRuby3ExpressionMutationF102Y.Y137LPromoterSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA6-mRuby3
Plasmid#184892PurposeStable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cellsDepositorInsertANXA6 (ANXA6 Human)
UseTagsmRuby3ExpressionMammalianMutationR587KPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE
Plasmid#196677PurposeAAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).DepositorInsertPKIα-P2A-mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-mRuby3
Plasmid#74252PurposeMammalian expression of tandem fusion of mClover3-mRuby3DepositorInsertmClover3-mRuby3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE
Plasmid#196678PurposeAAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.DepositorInsertPKIαmut-P2A-mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
GALT-SEP-mRuby3
Plasmid#200938Purposetrans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
MGAT-SEP-mRuby3
Plasmid#200937Purposecis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA11-mRuby3
Plasmid#184894PurposeStable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cellsDepositorInsertANXA11 (ANXA11 Human)
UseTagsmRuby3ExpressionMammalianMutationR230C relative to NP_001148.1PromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-SEP-mRuby3
Plasmid#200939Purposeendolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA1-mRuby3
Plasmid#184888PurposeStable expression of ANXA1-mRuby3 (Annexin A1-mRuby3) in mammalian cellsDepositorInsertANXA1 (ANXA1 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-mRuby3-FTH1
Plasmid#192804PurposeStable expression of mRuby3-FTH1 in mammalian cellsDepositorInsertFTH1 (FTH1 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV mRuby3-Synapsin1a
Plasmid#187896PurposeFor labeling pre-synaptic terminalsDepositorInsertSynapsin1a
UseTagsmRuby3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA5-mRuby3
Plasmid#184891PurposeStable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cellsDepositorInsertANXA5 (ANXA5 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only