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miniCGBE1-NG (pBM1182)

Plasmid

#140257

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

Plasmid

#109428

Purpose

A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry T2A GFP

Use

Lentiviral

Mutation

Inserted 43 base pairs into mCherry to create rep…

Promoter

CMV

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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miniCGBE1-VRQR (pBM1173)

Plasmid

#140255

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)_VRQR-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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SECURE BE4max(R33A) (pBM608)

Plasmid

#140251

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Tags

His6

Expression

Bacterial

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HF-BE3

Plasmid

#87439

Purpose

Expresses HF-BE3 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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CGBE1-VRQR (pBM1192)

Plasmid

#140254

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_VRQR-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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