We narrowed to 443 results for: DHFR
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Plasmid#85790PurposeP. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLIDepositorInsertPF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
UseP. falciparum genomic integrationTags2xFKBP, GFPPromoternoneAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLI-N-GFP-2xFKBP-loxP(K13)
Plasmid#85792PurposeP. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLIDepositorInsertKelch13
UseCre/Lox; P. falciparum genomic integrationTags2xFKBP, GFPPromoternoneAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-ZFP57
Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
SMT205
Plasmid#134817PurposeSMT201 with mutated RBS for DHFR, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
TagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyExpressionMammalianPromoterEf1alphaAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
strep-IRF8
Plasmid#102872PurposeExpress strep-tagged mouse IRF8 protein in E. coliDepositorAvailable SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-CTCF(F1-F9)
Plasmid#102859PurposeExpress DNA binding domain of mouse CTCF in E. coliDepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLI-N-sandwich-loxP(K13)
Plasmid#85793PurposeP. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLIDepositorInsertKelch13
UseCre/Lox; P. falciparum genomic integrationTagsdouble 2xFKBP, GFPPromoternoneAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-Gli1
Plasmid#87225PurposeExpress hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coliDepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-mCh-KDEL
Plasmid#173010PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.DepositorInsert2xFKBP-mCh-KDEL
TagsmCherryExpressionMammalianPromoterChicken beta actin (CAG)Available SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-smFP(myc)-KDEL
Plasmid#173021PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.DepositorInsert2xFKBP-smFP(myc)-KDEL
TagsFKBP and smFP(myc) non-fluorescentExpressionMammalianAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
TagsUbiquitin N-terminal fusion and triple flag tag C…ExpressionYeastMutationChanged first 4 N-terminal amino acid residues of…PromoterTDH3tc3Available SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHD22y/PfPRMT5-PTP
Plasmid#203160Purposefor tagging PTP to the 5' end of PfPRMT5 gene by single crossoverDepositorInsertPfPRMT5 fragment, PTP tag and pDT 3' UTR
Tags3' UTR of P. berghei dihydrofolate reductase…ExpressionMammalianPromoterNoAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD22Y/PfPRMT5 deletion
Plasmid#203161Purposefor disruption of PfPRMT5 geneDepositorInsertPfPRMT5 fragment
Tags3 myc tags and 3' UTR of P. berghei dihydrof…ExpressionMammalianPromoterNoAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
3'-ecRESCUE-mCherry
Plasmid#191486PurposeecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherryDepositorUseCRISPRExpressionMammalianPromoterCMVAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRHD-TetR-GAL80-Rho1-GAL4
Plasmid#218274PurposeIntroduction of low-temperature-inducible GAL system, LowTempGAL v2.2DepositorInsertgal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
ExpressionYeastMutationDHFR (P66L), Rho1 (C17R)Available SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only