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We narrowed to 555 results for: DHFR

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Showing: 381 - 400 of 555 results

  2. pAAH3

    Plasmid
    #41827
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41827-plasmid-map-sequence-id-69168

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  13. SMT215

    Plasmid
    #134818
    Purpose
    SMT205 with DHFR-FLAG-tag, western blot
    Depositor
    Tanja Kortemme
    Publication
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    134818-plasmid-map-sequence-id-313944

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  15. pAAH4

    Plasmid
    #41828
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41828-plasmid-map-sequence-id-69199

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  16. 2xFKBP-mCh-KDEL

    Plasmid
    #173010
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xFKBP-mCh-KDEL
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173010-plasmid-map-sequence-id-339203

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  18. 2xFKBP-smFP(myc)-KDEL

    Plasmid
    #173021
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xFKBP-smFP(myc)-KDEL
    Tags
    FKBP and smFP(myc) non-fluorescent
    Expression
    Mammalian
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173021-plasmid-map-sequence-id-339210

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  19. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102886-plasmid-map-sequence-id-194712

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  20. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Publication
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Expression
    Yeast
    Mutation
    DHFR P66L
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

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Showing: 381 - 400 of 555 results