We narrowed to 14,583 results for: pET

1. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176577

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-anticalin-FLN-ELP-His-ybbr

   Plasmid

   #168039

   Purpose

   Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SdrG-FLN-ELP-His-ybbr

   Plasmid

   #168047

   Purpose

   Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Use

   Tags

   3xELP linker, 6x Histag, ddFLN4, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-Synapsin-YPET-3XNLS-optoNES-WPRE

   Plasmid

   #159947

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   YPET-3XNLS-optoNES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Synapsin-NLS-YPET-optoNES-WPRE

   Plasmid

   #159942

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

   Plasmid

   #159975

   Purpose

   optical regulation of nuclear export with shorter relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.F434L
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   F434L

   Promoter

   hSyn1

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

   Plasmid

   #159973

   Purpose

   optical regulation of nuclear export with extended relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.V416I
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   V416I

   Promoter

   hSyn1

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

   Plasmid

   #159974

   Purpose

   optical regulation of nuclear export with shorter relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.I427V
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   I427V

   Promoter

   hSyn1

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-45b-TBP(38Q)-IDR-mCherry

    Plasmid

    #145283

    Purpose

    Bacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(38Q)-IDR (TBP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

    Plasmid

    #153443

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a human Tankyrase2 full length Y920E

    Plasmid

    #132639

    Purpose

    expresses human Tankyrase2 full length Y920E in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

    Insert

    Tankyrase2 Y920E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a asyn Q79N Q109N (TAC-->TAT)

    Plasmid

    #105738

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q79N/Q109N

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET151-Vps4(101-437)-Hcp1 K321D

    Plasmid

    #89288

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Asp

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET151-Vps4(101-437)-Hcp1 R325D

    Plasmid

    #89289

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Asp

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET151-Vps4(101-437)-Hcp1 E322A

    Plasmid

    #87748

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Glu-322 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET151-Vps4(101-437)-Hcp1 R325A

    Plasmid

    #87749

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET151-Vps4(101-437)-Hcp1 K321A

    Plasmid

    #87747

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

    Plasmid

    #61940

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156) GSGS (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 97-100 (N…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET-22HT-MSI1(7-192)R53E/R61E

    Plasmid

    #60354

    Purpose

    R53E R61E double mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E and R61E mutations; contains amino acids 7-1…

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only