We narrowed to 5,111 results for: codon optimized

1. pYPQ230-RVR

   Plasmid

   #108861

   Purpose

   LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G548R, K554V and Y558R, Cpf1 is rice codon optimi…

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-SFXN2

   Plasmid

   #132256

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SFXN2 (SFXN2 Human)
   

   Expression

   Mammalian

   Available Since

   March 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDONR221_SLC26A11

   Plasmid

   #131996

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC26A11 (SLC26A11 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDONR221_SLC24A3

   Plasmid

   #131978

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC24A3 (SLC24A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

   Plasmid

   #209851

   Purpose

   Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

   Depositor

   Volker Busskamp

   Article

   Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

   Insert

   light-driven sodium pump ErNaR
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-mCherry-C1-Aurora_CA

   Plasmid

   #98170

   Purpose

   slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Aurora_C128A gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

   Promoter

   CMV (+enhancer)

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC12A7

   Plasmid

   #131873

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC12A7 (SLC12A7 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDONR221_SLC22A23

   Plasmid

   #131988

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A23 (SLC22A23 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR221_SLC4A5

   Plasmid

   #132284

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC4A5 (SLC4A5 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Nsp13 K289A-EGFP

    Plasmid

    #165130

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp13 K289A (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221_SLC9A5

    Plasmid

    #132163

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC9A5 (SLC9A5 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid

    #198569

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221_SLC26A7

    Plasmid

    #132152

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC26A7 (SLC26A7 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139097

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dxCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. hSyn1-AsCpf1(RR)

    Plasmid

    #109325

    Purpose

    AAV vector expressing AsCpf1 (RR variant)

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    hAsCpf1(RR)
    

    Use

    AAV

    Tags

    HA and NLS

    Mutation

    S542R/K607R

    Promoter

    hSyn1

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221_SLC15A2

    Plasmid

    #131886

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC15A2 (SLC15A2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDONR221_SLC26A6

    Plasmid

    #132056

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC26A6 (SLC26A6 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pEE062

    Plasmid

    #176819

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    701
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJJGL004

    Plasmid

    #180608

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    10xHis and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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