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NOLC1 nicking guide pDY2251

Plasmid

#219861

Purpose

Nicking sgRNA for NOLC1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 nicking sgRNA (NOLC1 Human)

Use

CRISPR

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Rab4a-C-7

Plasmid

#231781

Purpose

Visualization of endosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Rab4a-C-7

Plasmid

#231773

Purpose

Visualization of endosome in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP50

Plasmid

#196788

Purpose

Contains ACS2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE 2 (ACS2 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

150 C->G

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP51

Plasmid

#196789

Purpose

Contains AOS promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

ALLENE OXIDE SYNTHASE (AOS Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

2088 G->C ; 1434 G->C

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP55

Plasmid

#196793

Purpose

Contains PDF1.2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PLANT DEFENSIN 1.2 (PDF1.2 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP56

Plasmid

#196794

Purpose

Contains PR1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PATHOGENESIS-RELATED GENE 1 (PR1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP27

Plasmid

#196765

Purpose

Contains CPK5 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

CALMODULIN-DOMAIN PROTEIN KINASE 5 (CPK5 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP30

Plasmid

#196768

Purpose

Contains GLR2.7 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

GLUTAMATE RECEPTOR 2.7 (GLR2.7 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP36

Plasmid

#196774

Purpose

Contains DORN1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

DOES NOT RESPOND TO NUCLEOTIDES 1 (LecRK-I.9 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP41

Plasmid

#196779

Purpose

Contains WRKY33 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

WRKY DNA-BINDING PROTEIN 33 (WRKY33 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP14

Plasmid

#196752

Purpose

Contains LYM1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

LYSM DOMAIN GPI-ANCHORED PROTEIN 1 PRECURSOR (LYM1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2259 hNOLC1 atgRNA Paired Guide 1

Plasmid

#220989

Purpose

hNOLC1 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2260 hNOLC1 atgRNA Paired Guide 2

Plasmid

#220990

Purpose

hNOLC1 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2261 hACTB atgRNA Paired Guide 1

Plasmid

#220991

Purpose

hACTB atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

ACTB atgRNA (ACTB Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2262 hACTB atgRNA Paired Guide 2

Plasmid

#220992

Purpose

hACTB atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

ACTB atgRNA (ACTB Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16A-I547K-peGFP-N1

Plasmid

#228583

Purpose

Expresses mouse TMEM16A-I547K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)

Expression

Mammalian

Mutation

I547K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16A-E551K-peGFP-N1

Plasmid

#228584

Purpose

Expresses mouse TMEM16A-E551K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)

Expression

Mammalian

Mutation

E551K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-Venus-Rab11

Plasmid

#228140

Purpose

Expresses Venus-Rab11 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab11 (RAB11A Canis lupus familiaris)

Use

Retroviral

Tags

Venus

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-MAAP-SL01V6

Plasmid

#218249

Purpose

1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein Variant SL01V6 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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