We narrowed to 4,156 results for: pes

1. LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZ

   Plasmid

   #192384

   Purpose

   To act as the on state for testing of the alt B3 recombinase.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-OCS-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP716_L2sV3_NAND_1IF2_FRT_Rluc_FlpF2_C1_lacZ

   Plasmid

   #192416

   Purpose

   To act as a 1-input state of the split-NAND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::RlucRluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP717_L2sV3_NAND_2I_FRT_Rluc_Flp-C1_lacZ

   Plasmid

   #192417

   Purpose

   To act as the 2-input state of the split-NAND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::RlucRluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP715_L2sV3_NAND_1IF1_FRT_Rluc_FlpF1_C1-NLS_lacZ

   Plasmid

   #192415

   Purpose

   To act as a 1-input state of the split-NAND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::RlucRluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1

   Plasmid

   #192414

   Purpose

   To act as the 2-input state of the split-AND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP764_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IB_lacZ

   Plasmid

   #192408

   Purpose

   To act as a 1-input state of the A NIMPLY B gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCfB9341 (pgRNA_IX-1_NatMX)

   Plasmid

   #161593

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB9343 (pgRNA_XV-1_NatMX)

   Plasmid

   #161595

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MTK4a_021

   Plasmid

   #123834

   Purpose

   Encodes a nuclear localized and destabilized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   T2A::NLS::mRuby2::PEST4d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.3_d2eGFP

    Plasmid

    #26821

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-d2eGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    destabilized (PEST sequence) GFP

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. N8his-GFPenhancer-GGGGS4-LaG16

    Plasmid

    #140442

    Purpose

    We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

    Depositor

    Motoyuki Hattori

    Article

    Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

    Insert

    GFPenhancer-GGGGS4-LaG16
    

    Use

    Tags

    8 histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-REPORT (PGK) 12xHRE-NanoLuc

    Plasmid

    #208575

    Purpose

    hypoxic response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    12xHRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEMS-II-dCas13-APO1-YTH

    Plasmid

    #204767

    Purpose

    GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    dCas13-NLS-APO1-YTH-HA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1alpha

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUCmini-iCAP-PHP.AX

    Plasmid

    #195218

    Purpose

    AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

    Depositor

    Viviana Gradinaru

    Article

    Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

    Insert

    Synthetic construct isolate AAV-PHP.AX VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    35S

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. dCas9-10xSunTag(22aa)

    Plasmid

    #215745

    Purpose

    Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    dCas9-10xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLQ1371-sgSTAT3-1

    Plasmid

    #121425

    Purpose

    sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgSTAT3-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HIV-dual-GT

    Plasmid

    #122696

    Purpose

    Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

    Depositor

    Klaus Überla

    Article

    Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

    Inserts

    mTagBFP2
    DsRed-max
    

    Use

    Lentiviral

    Tags

    PEST domain

    Expression

    Mutation

    Promoter

    HIV-1 LTR

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2_Firefly_degron-60A

    Plasmid

    #200396

    Purpose

    A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

    Depositor

    Xiao Wang

    Article

    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

    Insert

    Firefly luciferase - degron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTK331

    Plasmid

    #59572

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge