We narrowed to 44,102 results for: cha
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Plasmid#228966PurposeFor bacterial expression of anti-GFP nanobody LaG94-6, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-6
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-18
Plasmid#228972PurposeFor bacterial expression of anti-GFP nanobody LaG94-18, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-18
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-2
Plasmid#228975PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-2
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaGIgG-4
Plasmid#228998PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-4, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaGIgG-4
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-2
Plasmid#228962PurposeFor bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-2
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-1
Plasmid#228961PurposeFor bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-9
Plasmid#228993PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-9
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-8
Plasmid#228992PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-8
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-10
Plasmid#228978PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-10
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-4
Plasmid#228964PurposeFor bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-4
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-8
Plasmid#228976PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-8
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-9
Plasmid#228977PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-9
UseTags6xHISExpressionBacterialMutationPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ 3xFLAG-FEM1B WT
Plasmid#230993PurposeTransient overexpression of mouse FEM1B in mammalian cellsDepositorAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh2CDS
Plasmid#192841PurposePlasmid to produce ISH probe for mMyh2 gene with CDS sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh2UTR
Plasmid#192842PurposePlasmid to produce ISH probe for mMyh2 gene with UTR sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh3CDS
Plasmid#192843PurposePlasmid to produce ISH probe for mMyh3 gene with CDS sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh3UTR
Plasmid#192844PurposePlasmid to produce ISH probe for mMyh3 gene with UTR sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh4CDS
Plasmid#192845PurposePlasmid to produce ISH probe for mMyh4 gene with CDS sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh4UTR
Plasmid#192846PurposePlasmid to produce ISH probe for mMyh4 gene with UTR sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh6CDS
Plasmid#192847PurposePlasmid to produce ISH probe for mMyh6 gene with CDS sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh6UTR
Plasmid#192848PurposePlasmid to produce ISH probe for mMyh6 gene with UTR sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh8UTR
Plasmid#192852PurposePlasmid to produce ISH probe for mMyh8 gene with UTR sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46-His (SB250)
Plasmid#227599PurposeInducible expression of His-tagged SLC25A46 in Pichia pastorisDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)
Plasmid#227614PurposeOverexpression of SLC25A46 in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
UseTagsP2A-mElectra1ExpressionMammalianMutationPromoterCMV, T7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL01V6
Plasmid#218249Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL01V6 driven by CMV promoter
UseLentiviralTagsExpressionMutationPromoterCMVAvailable SinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL01V9
Plasmid#218250Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL01V9 driven by CMV promoter
UseLentiviralTagsExpressionMutationPromoterCMVAvailable SinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL08V9
Plasmid#218251Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter
UseLentiviralTagsExpressionMutationPromoterCMVAvailable SinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL01
Plasmid#218248Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL01 driven by CMV promoter
UseLentiviralTagsExpressionMutationPromoterCMVAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225649PurposetetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA, araBAD and LacO3O1 in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromotertetA, araBAD and LacO3O1 promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-FIVAR-ELP-HIS-ybbR
Plasmid#169136PurposeE. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertFIVAR (found in various architectures)
UseTags3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…ExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-VHH-ddFLN4-HIS-ybbR
Plasmid#169137PurposeE. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertVHH
UseTags6x His tag, ddFLN4 tag, and ybbr tagExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(Q258K)
Plasmid#224763PurposeIn vitro transcription for Oocyte expression of hTRAAK(Q258K)DepositorInsertPotassium channel subfamily member 4 (KCNK4 Human)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationQ258KPromoterT7Available SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1
Plasmid#224743PurposeIn vitro transcription for Oocyte expression of mTREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationPromoterT7Available SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK
Plasmid#224761PurposeIn vitro transcription for Oocyte expression of hTRAAKDepositorInsertPotassium channel subfamily member 4 (KCNK4 Human)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationPromoterT7Available SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(S118C)
Plasmid#224762PurposeIn vitro transcription for Oocyte expression of hTRAAK(S118C)DepositorInsertPotassium channel subfamily member 4 (KCNK4 Human)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS118CPromoterT7Available SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTRAAK
Plasmid#224765PurposeIn vitro transcription for Oocyte expression of mTRAAKDepositorInsertPotassium channel subfamily member 4 (Kcnk4 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationPromoterT7Available SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pt NaV1.4-N1600T
Plasmid#200289PurposeExpression in mammalian cells or oocytesDepositorInsertPhyllobates terriblis NaV1.4 – muscle channel
UseTagsExpressionMammalianMutationN1600TPromoterCMV and T7Available SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dt NaV1.4-N1600T
Plasmid#200308PurposeExpression in mammalian cells or oocytesDepositorInsertDendrobates tinctorius NaV1.4 – muscle channel
UseTagsExpressionMammalianMutationN1600TPromoterCMV and T7Available SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEndo-I-OnuI-TSM
Plasmid#207954PurposeExpresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.DepositorInsertThermosensitive fusion of I-OnuI and the L212P VMA1 intein.
UseSynthetic BiologyTagsExpressionBacterialMutationLeucine 212 changed to proline, leucine 434 in fu…PromoterAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
Plasmid#218766Purposeencodes EcTyrRS-VSMA* and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-VSMA*
UseTagsExpressionBacterialMutationY37V, D167G, D182S, F183M, L186A, D265RPromotertacIAvailable SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
UseTagsExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP
Plasmid#207025PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371A I375A D384A Y387APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Pho8-406
Plasmid#207006PurposeIntegration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.DepositorInsertPHO8
UseTagsGFPExpressionYeastMutationPromoterAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
UseTagsExpressionYeastMutationPromoterAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Osh1-406
Plasmid#207007PurposeIntegration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.DepositorInsertOSH1
UseTagsGFPExpressionYeastMutationPromoterAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only