We narrowed to 4,089 results for: PRS

1. pCMV-mKO2-Parkin

   Plasmid

   #213544

   Purpose

   Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Parkin(E16A)

   Plasmid

   #213546

   Purpose

   Transient mammalian expresssion of E16A Parkin

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E16A mutation in Parkin

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. W118-1_eGFP

   Plasmid

   #180379

   Purpose

   lentiviral transduction of eGFP gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   eGFP (eGFP )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCu-His6-Atg13[571–700]-Ss(424)

   Plasmid

   #166852

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   pCu

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CPH3486 (YCp LEU2 Rpb1 F1492A)

   Plasmid

   #91808

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed phenylalanine 1492 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CPH3487 (YCp LEU2 Rpb1 S1493A)

   Plasmid

   #91809

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed serine 1493 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CPH3488 (YCp LEU2 Rpb1 P1494A)

   Plasmid

   #91810

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed proline 1494 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CPH3508 (YCp LEU2 Rpb1 Y1473A)

   Plasmid

   #91811

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed tyrosine 1473 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CPH3509 (YCp LEU2 Rpb1 T1471A)

   Plasmid

   #91812

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed threonine 1471 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CPH3511 (YCp LEU2 Rpb1 P1472A)

    Plasmid

    #91813

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed proline 1472 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBB0238-TEV-His12

    Plasmid

    #137038

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLC-Flag-SENP8 sg2R-P2A-Hygro

    Plasmid

    #124300

    Purpose

    Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    SENP8 (SENP8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCu-GFP-Atg17(416) (L313D I314K)

    Plasmid

    #121409

    Purpose

    Expressing GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-GFP-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 313 to Aspartic acid; Isoleucine 314 to …

    Promoter

    CUP1 promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PRPS2 gRNA (BRDN0001145436)

    Plasmid

    #78008

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRPS2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRPS2 (PRPS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PRPS2 gRNA (BRDN0001146576)

    Plasmid

    #78007

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRPS2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRPS2 (PRPS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcBac1-EcTrp-TGA

    Plasmid

    #228780

    Purpose

    aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-MBP human SPOP (aa 28-337)

    Plasmid

    #52294

    Purpose

    bacterial expression of human SPOP (aa 28-337) fused to His-MBP

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    SPOP (SPOP Human)
    

    Use

    Tags

    His-MBP and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid

    #182395

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & Leucine Zi…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHtrA-TEV-His12

    Plasmid

    #137036

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OCT4-eGFP-2A-Puro

    Plasmid

    #31939

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Inserts

    OCT4 (POU5F1 Human)
    eGFP (eGFP )
    

    Use

    Targeting donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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