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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 37,838 results for: KAN

Showing: 10101 - 10150 of 37838 results

  1. mRuby-Endo-14

    Plasmid
    #55859
    Purpose
    Localization: Endosomes, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Endo (RHOB Human)
    Use
    Tags
    mRuby and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. FLAG-1858T-PTPN22

    Plasmid
    #195378
    Purpose
    Mammalian expression of a human PTPN22 mutant (1858T) with FLAG tag
    Depositor
    Patrick Concannon
    Insert
    PTPN22 (PTPN22 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    T at rs2476601 (C>T)
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195378-plasmid-map-sequence-id-386962

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  3. tdEos-Occludin-N-10

    Plasmid
    #57651
    Purpose
    Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. mRuby-Zyxin-6

    Plasmid
    #55887
    Purpose
    Localization: Focal Adhesions, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Zyxin (ZYX Human)
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    S11P, G42R, E62D compared to NM_003461
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55887-plasmid-map-sequence-id-357016

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  6. tdTomato-Vinculin-23

    Plasmid
    #58145
    Purpose
    Localization: Focal Adhesions, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    V234L in Vinculin
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. mApple-PDHA1-N-10

    Plasmid
    #54938
    Purpose
    Localization: Mitochondria, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    PDHA1 (PDHA1 Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pLK89 - Edit-directing plasmid cloning insert

    Plasmid
    #98846
    Purpose
    Contains the insert used for step 2 of edit-directed plasmid cloning
    Depositor
    Leonid Kruglyak
    Insert
    KanR
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Changed sequence to remove a MluI cut site
    Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98846-plasmid-map-sequence-id-189230

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  9. pT7-V5-SBP-C1-HsYTHDF3_AB

    Plasmid
    #148326
    Purpose
    Mammalian Expression of HsYTHDF3
    Depositor
    Elisa Izaurralde
    Insert
    HsYTHDF3 (YTHDF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148326-plasmid-map-sequence-id-396937

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  11. mCerulean-H4-23

    Plasmid
    #55378
    Purpose
    Localization: Nucleus/Histones, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    H4 (HIST1H4J Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55378-plasmid-map-sequence-id-233119

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  12. EYFP-Endosomes-13

    Plasmid
    #56588
    Purpose
    Localization: Endosomes, Excitation: 513, Emission: 527
    Depositor
    Michael Davidson
    Insert
    Endosomes (RHOB Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. 6P0Q

    Plasmid
    #162262
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR12 (WDR12 Human)
    Use
    Tags
    TEV-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162262-plasmid-map-sequence-id-319829

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  14. mKate-H4-23

    Plasmid
    #56061
    Purpose
    Localization: Nucleus/Histones, Excitation: 488, Emission: 635
    Depositor
    Michael Davidson
    Insert
    H4 (HIST1H4J Human)
    Use
    Tags
    mKate
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. Nup54-mEGFP494(flex0)

    Plasmid
    #163429
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 5 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163429-plasmid-map-sequence-id-321671

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  16. pDD373

    Plasmid
    #137021
    Purpose
    SapTrap GFP-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized GFP
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137021-plasmid-map-sequence-id-274046

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  17. PS-CFP2-PCNA-19-SV40NLS-4

    Plasmid
    #57245
    Purpose
    Localization: Membrane Scaffold, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Use
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57245-plasmid-map-sequence-id-178439

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  18. LCB7_crtYB

    Plasmid
    #140232
    Purpose
    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene
    Depositor
    Kang Zhou
    Insert
    phytoene-beta carotene synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K307E
    Promoter
    pthrC3
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    140232-plasmid-map-sequence-id-288146

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  20. tdEos-MyosinIIB-C-18

    Plasmid
    #57646
    Purpose
    Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    MyosinIIB (MYH10 Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. mEos2-Vinculin-N-21

    Plasmid
    #57439
    Purpose
    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pT7-V5-SBP-C1-HshnRNPE2iso3_Z

    Plasmid
    #148140
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148140-plasmid-map-sequence-id-385303

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  23. mKOet-TFR-20

    Plasmid
    #57935
    Purpose
    Localization: Transferrin Receptor, Excitation: 548, Emission: 561
    Depositor
    Michael Davidson
    Insert
    TFR (TFRC Human)
    Use
    Tags
    mKOet
    Expression
    Mammalian
    Mutation
    G142S
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    57935-plasmid-map-sequence-id-241031

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  24. PS-CFP2-MyosinIIB-C-18

    Plasmid
    #57242
    Purpose
    Localization: Mitochondria, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    MyosinIIB (MYH10 Human)
    Use
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pETM-30-GST-Dmp68_B

    Plasmid
    #145962
    Purpose
    Bacterial Expression of Dmp68
    Depositor
    Elisa Izaurralde
    Insert
    Dmp68 (Rm62 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145962-plasmid-map-sequence-id-382819

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  27. mTurquoise-PDHA1-N-10

    Plasmid
    #55574
    Purpose
    Localization: Mitochondria, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    PDHA1 (PDHA1 Human)
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pAG472 H2B-iFAST

    Plasmid
    #130818
    Purpose
    Expresses H2B-iFAST in mammalian cells (nuclear labeling)
    Depositor
    Arnaud Gautier
    Insert
    H2B-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130818-plasmid-map-sequence-id-254956

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  29. Nup54-mEGFP494(flex2)

    Plasmid
    #163431
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 7 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163431-plasmid-map-sequence-id-321673

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  30. Dronpa-PDHA1-N-10

    Plasmid
    #57292
    Purpose
    Localization: Mitochondria, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    PDHA1 (PDHA1 Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pJK402

    Plasmid
    #72415
    Purpose
    Produces Acetobacter aceti 1023 Est03, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    4-hydroxybenzoyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72415-plasmid-map-sequence-id-135527

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  32. mRFP1-Vinculin-23

    Plasmid
    #55924
    Purpose
    Localization: Focal Adhesions, Excitation: 584, Emission: 607
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    mRFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  33. PA-mCherry1-SiT-N-15

    Plasmid
    #57197
    Purpose
    Localization: Golgi TGN, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    SiT (ST6GAL1 Human)
    Use
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Mutation
    aa 1-45 of SiT
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. PA-mCherry1-Vinculin-23

    Plasmid
    #57203
    Purpose
    Localization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. mEos2-MyosinIIB-N-18

    Plasmid
    #57404
    Purpose
    Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    MyosinIIB (MYH10 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. TBRII-N-18

    Plasmid
    #58196
    Purpose
    Localization: Membrane Receptor, Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    TBRII (TGFBR2 Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58196-plasmid-map-sequence-id-173163

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  37. mEmerald-Tubulin-N-18

    Plasmid
    #54293
    Purpose
    Localization: Microtubules, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. Paxillin_PSCFP2_ mEOS2

    Plasmid
    #114192
    Purpose
    studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling
    Depositor
    Aleksandra Radenovic
    Insert
    Paxillin (PXN Chicken)
    Use
    Tags
    PS-CFP2 and mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114192-plasmid-map-sequence-id-225613

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  39. pENTRY4_ Pi21

    Plasmid
    #126904
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126904-plasmid-map-sequence-id-249827

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  40. mAzurite-Vinculin-23

    Plasmid
    #55234
    Purpose
    Localization: Focal Adhesions, Excitation: 384, Emission: 450
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    mAzurite
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  41. mEos3.2-TES-N-14

    Plasmid
    #57482
    Purpose
    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    TES (TES Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57482-plasmid-map-sequence-id-213111

    Enlarge

  42. mApple-Endo-14

    Plasmid
    #54894
    Purpose
    Localization: Endosomes, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Endo (RHOB Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54894-plasmid-map-sequence-id-173162

    Enlarge

  43. UBC45

    Plasmid
    #25469
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc45.001.200
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  44. Clim-DC1-mCherry-H2B-Venus

    Plasmid
    #129559
    Purpose
    nuclear marker
    Depositor
    Inaki Ruiz-Trillo
    Insert
    mcherry and Histone 2B fused to venus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129559-plasmid-map-sequence-id-258976

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  45. Nup54-mEGFP494(flex1)

    Plasmid
    #163430
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 6 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163430-plasmid-map-sequence-id-321672

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  46. Nup54-mEGFP494(1)

    Plasmid
    #163427
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163427-plasmid-map-sequence-id-321664

    Enlarge

  47. mWasabi-Endo-14

    Plasmid
    #56500
    Purpose
    Localization: Endosomes, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Endo (RHOB Human)
    Use
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. 4ROJ

    Plasmid
    #64661
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    VAV2:JMC092-C08:C231327 (VAV2 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    May 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. mEmerald-Talin-ABM-10

    Plasmid
    #54268
    Purpose
    Localization: Talin Actin Binding Domain, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Talin-ABM
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Aactin Binding Module; I/LWEQ = 197 amino acids f…
    Promoter
    CMV
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  50. NUDT14A

    Plasmid
    #42485
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT14A
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 10101 - 10150 of 37838 results