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pBEL2080

Plasmid

#155160

Purpose

Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_L266A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2081

Plasmid

#155161

Purpose

Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF(Y261A_L265A_L266A)

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1305

Plasmid

#155143

Purpose

Expresses MlaFB, His tag on MlaF N-term

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(6xHis-TEV-MlaF)-MlaB

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1306

Plasmid

#155144

Purpose

Expresses MlaFB, His tag on MlaF C-term

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaF-6xHis)-MlaB

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1307

Plasmid

#155145

Purpose

Expresses MlaFB, His tag on MlaB N-term

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1308

Plasmid

#155146

Purpose

Expresses MlaFB, His tag on MlaB C-term

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(MlaB-6xHis)

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1833

Plasmid

#155149

Purpose

Expresses MlaF(1-250)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-250)-GCN4_Dimerization_Domain

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1957

Plasmid

#155151

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

added Streptavidin tag on MlaF N-terminus

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2074

Plasmid

#155155

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaB_T52E

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2076

Plasmid

#155156

Purpose

Expresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_K47A

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2077

Plasmid

#155157

Purpose

Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_E170Q

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2078

Plasmid

#155158

Purpose

Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_Y261A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2079

Plasmid

#155159

Purpose

Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_L265A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986C

Plasmid

#125000

Purpose

Expresses dCas9-VPR driven by the bottleneck promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

bottleneck

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986D

Plasmid

#125001

Purpose

Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Expression

Insect

Promoter

ubiquitin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986E

Plasmid

#125002

Purpose

Expresses dCas9-VP64 driven by the bottleneck promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Expression

Insect

Promoter

bottleneck

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK M15 (CUA)

Plasmid

#154784

Purpose

plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

G10A, G14U, G16U, C20U, C52A

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK M15 (UUA)

Plasmid

#154785

Purpose

plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

UUA anticodon and G10A, G14U, G16U, C20U, C52A

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050I (notch)

Plasmid

#132421

Purpose

express arrays of gRNA targeting Notch under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

notch gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050K (firefly luciferase)

Plasmid

#132422

Purpose

express arrays of gRNA targeting Firefly Luciferase under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

firefly Luciferase gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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