We narrowed to 10,320 results for: plasmids 101

1. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

   Plasmid

   #225587

   Purpose

   Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-vhhGFP4-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRAPPC8_pLX307

   Plasmid

   #98375

   Purpose

   Lentiviral expression of TRAPPC8

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   TRAPPC8 (TRAPPC8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

   Plasmid

   #188541

   Purpose

   Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-Idax WT

   Plasmid

   #68553

   Purpose

   This plasmid expresses full length IDAX.

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   IDAX (Cxxc4 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRuby2-Keratin-17

   Plasmid

   #55900

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   March 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pGL3-Cp110-3'UTR

   Plasmid

   #64083

   Purpose

   Luciferase assay

   Depositor

   Lin He

   Article

   Song et al Nature. 2014 Jun 5;510(7503):115-20. doi

   Insert

   Ccp110 (centriolar coiled coil protein 110) (Ccp110 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1 T21R-Nb139-T2A-mCherry

   Plasmid

   #225589

   Purpose

   Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-Nb139-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. phABCA3-eGFP_CRISPR_Donor

   Plasmid

   #188540

   Purpose

   Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   EGFP
   

   Use

   CRISPR; Donor for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. dre-cdk2 3'UTR sicheck2

   Plasmid

   #121123

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-cdk2 3'UTR (cdk2 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-U6-empty

    Plasmid

    #140580

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mpCHECK2

    Plasmid

    #167576

    Purpose

    The mpCHECK2 plasmid has the ability to evaluate the effect of polyadenylation signal variants on expression of disease-associated genes.

    Depositor

    Ting Ni

    Article

    Chen et al Genome Res. 2021 Apr 19. pii: gr.270256.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1 _Gata6

    Plasmid

    #73537

    Purpose

    Inducible lentiviral expression of Gata6

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Gata6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

    Plasmid

    #74947

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Oct3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Flag-Tet1 CD Mut

    Plasmid

    #72218

    Purpose

    Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET1 catalytic domain (cata…

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

15. HAP40 1-371

    Plasmid

    #124060

    Purpose

    Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HAP40 (F8A1 Human)
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBiFC-PBZ-VC

    Plasmid

    #110648

    Purpose

    expresses a PAR-binding (PBZ) domain fused to venus C-terminus

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Use

    Tags

    HA and VC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Flag-Tet2 CD Mut

    Plasmid

    #72220

    Purpose

    Express flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 2 (Tet2 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET2 catalytic domain (cata…

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

18. Duffy-CD4d3+4-bio

    Plasmid

    #73122

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Duffy with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Duffy (ACKR1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBItetDT240GFP

    Plasmid

    #96904

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
    

    Use

    Tetracycline responsive and bidirectional

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline responsive and bidirectional

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. JA740

    Plasmid

    #49939

    Purpose

    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD GFP (TET1 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only