We narrowed to 2,962 results for: ER

1. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

   Plasmid

   #197506

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

   Plasmid

   #106206

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: S72A + mRuby3 fusion

   Promoter

   CAG

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.SF-Venus-iGluSnFR.A184V

   Plasmid

   #106202

   Purpose

   Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-syn-FLEX-axon-jYCaMP1s

   Plasmid

   #135421

   Purpose

   Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Tags

   GAP43 axon targeting sequence

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-syn-FLEX-jYCaMP1s

   Plasmid

   #135420

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMThy1.1_hDDX5_FL_D248N

   Plasmid

   #88874

   Purpose

   constitutive expression of DDX5 D248N in mammalian cells

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D248N

   Promoter

   MSCV

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSR:MSMEG_0030:Dendra

   Plasmid

   #159040

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0030
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSR:MSMEG_5048:Dendra

   Plasmid

   #167366

   Purpose

   C-term fluorescent fusion

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_5048
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106203

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   CAG

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106183

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    hSynapsin

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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