We narrowed to 2,463 results for: LARGE

1. pAAV MP-135 mRFP

   Plasmid

   #206927

   Purpose

   The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.

   Depositor

   Markus Grompe

   Article

   Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

   Insert

   mRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MP-135

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Synapsin sHRPa-NRX in FSW lentiviral vector

   Plasmid

   #73147

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Lentiviral

   Tags

   2x HA tag and Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGPTVII-Bar-U-MatryoshCaMP6s

   Plasmid

   #100024

   Purpose

   Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.2-DEST-MatryoshCaMP6s

   Plasmid

   #100025

   Purpose

   Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Use

   Tags

   V5 Epitope Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM2AH + mSlo1

   Plasmid

   #16152

   Depositor

   Larry Salkoff

   Article

   Butler et al Science. 1993 Jul 9. 261(5118):221-4.

   Insert

   Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pDG330

   Plasmid

   #100898

   Purpose

   SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET a MatryoshCaMP6s

   Plasmid

   #100019

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2xFlag CMV2 Lats1-PY1 mutant

   Plasmid

   #18973

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Large tumor suppressor kinase 1 PY-1 mutant (LATS1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   PY1 mutant, Tyrosine 376 changed to Alanine

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

9. p2xFlag CMV2 Lats1-N-mutant

   Plasmid

   #18976

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Large tumor suppressor kinase 1, N mutant (LATS1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   N-mutant : truncated after aa 587

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

10. 1352wUV2 F2bbs kan GCANNNAGGk

    Plasmid

    #18752

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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