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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,245 results for: ACE;

Showing: 6091 - 6120 of 20245 results

  1. SpyTag003-OspC

    Plasmid
    #159998
    Purpose
    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells
    Depositor
    Mark Howarth
    Article
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Insert
    SpyTag003-OspC (20-209) C129S
    Use
    Tags
    His6-tag and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    C129S mutation improves expression level.
    Promoter
    T7
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159998-plasmid-map-sequence-id-316165

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  11. qTAG-C-Solo-mScarlet-H2BC11

    Plasmid
    #207756
    Purpose
    Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207756-plasmid-map-sequence-id-415157

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  17. pre_IV_CS10_1545_v1

    Plasmid
    #126861
    Purpose
    4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation
    Depositor
    Hendrik Dietz
    Article
    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn
    Insert
    IV_CS10_1545_v1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    126861-plasmid-map-sequence-id-250149

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  19. pASPIre1

    Plasmid
    #154842
    Purpose
    Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Prha
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154842-plasmid-map-sequence-id-307626

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  24. pASPIre3

    Plasmid
    #154844
    Purpose
    Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154844-plasmid-map-sequence-id-307642

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  27. KTK_354

    Plasmid
    #180525
    Purpose
    KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180525-plasmid-map-sequence-id-358850

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  29. pAV115

    Plasmid
    #63182
    Purpose
    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63182-plasmid-map-sequence-id-104856

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  30. pCK314

    Plasmid
    #110545
    Purpose
    Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    Modified PrhaBAD (CRP-binding sites removed)
    yfp
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110545-plasmid-map-sequence-id-212174

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Showing: 6091 - 6120 of 20245 results