We narrowed to 3,002 results for: ALC

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pPRISM-Stop-cmlc2-TagBFP

Plasmid

#117774

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGBT-RP8.1

Plasmid

#189846

Purpose

Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.

Depositor

Stephen Ekker

Article

Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

Type

Empty backbone

Use

Cre/Lox

Tags

mRFP and tagBFP

Promoter

beta Actin

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-gcry-TagBFP

Plasmid

#117783

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-Nix-CytoB5

Plasmid

#100756

Purpose

Mammalian expression of ER targeted Nix

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Nix-CytoB5 (BNIP3L Human)

Tags

Myc

Expression

Mammalian

Mutation

TM domain replaced by CytoB

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-gcry-TagBFP

Plasmid

#117789

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a_crRNA NC

Plasmid

#176256

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Mutation

D917A and E1006A mutations to inactivate the endo…

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-W318R

Plasmid

#174793

Purpose

Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-W318R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

W318, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-eGFP

Plasmid

#117788

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/E2F2/NRF-1(-64)-like

Plasmid

#66742

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCG(-64) to TACA

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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