We narrowed to 10,226 results for: Coli
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Plasmid#120225PurposepCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.DepositorInsertSpCas9-NG
UseSynthetic BiologyTagsssrAMutationMutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…PromoterpTetAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET mRuby2 LIC cloning vector (H6-mRuby2)
Plasmid#86934PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mR…ExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB355
Plasmid#22070DepositorTypeEmpty backboneTagsT18ExpressionBacterialAvailable SinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pExp-PpiB
Plasmid#112572PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-PpiB and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2
Plasmid#177231PurposeExpresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinaseDepositorInsertsgNeo1/sgNT1/sgNeo2
UseLentiviralPromoterbU6/mU6/hU6Available SinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET mClover LIC cloning vector (H6-mClover)
Plasmid#86931PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mCloverDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9CR4-VQR
Plasmid#120224PurposepCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015DepositorInsertSpCas9-VQR
UseSynthetic BiologyTagsssrAMutationMutations D1135V/R1335V/T1337RPromoterpTetAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExp-DsbA(SS)
Plasmid#112563PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-DsbA(SS) and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -