Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,474 results for: BLI;

Clear keywords
Showing: 8161 - 8200 of 9474 results

  4. pAAV-hsyn-DIO-AppNL-G-F-T2A-mCherry

    Plasmid
    #242649
    Purpose
    Floxed (DIO) AAV transgene designed to be packaged in AAV and to express the amyloid precursor protein (App) with familial mutations AppNL-G-F in a Cre-recombinase-dependent manner.
    Depositor
    William Wisden
    Insert
    Floxed (DOI) AAV transgene expressing an amyloid precursor protein with familial mutations and an mCherry tag
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Mutation
    The amyloid precursor reading frame contains a c…
    Promoter
    human synapsin
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    242649-plasmid-map-sequence-id-489352

    Enlarge

  5. SmBiT-KRAS(G12V) BiBiT Ready Vector

    Plasmid
    #238832
    Purpose
    Express SmBiT-KRAS(G12V) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238832-plasmid-map-sequence-id-479923

    Enlarge

  6. NanoBRET Assay Vector, NanoLuc-KRas(G12D)

    Plasmid
    #238581
    Purpose
    Express NanoLuc-KRas(G12D) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238581-plasmid-map-sequence-id-481209

    Enlarge

  7. NanoBRET Assay Vector, HaloTag-KRAS(G12V)

    Plasmid
    #238570
    Purpose
    Express HaloTag-KRAS(G12V) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238570-plasmid-map-sequence-id-485890

    Enlarge

  8. pEmal10H – MBP-EWS_300-447 (RGG1+RRM)

    Plasmid
    #238369
    Purpose
    Expresses the RGG1-RRM (300-447) region from the RNA-binding protein EWS
    Depositor
    David Libich
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-299, 448-638
    Promoter
    T7
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238369-plasmid-map-sequence-id-478743

    Enlarge

  9. pEmal10H – MBP-EWS_549-638 (RGG3)

    Plasmid
    #238370
    Purpose
    Expresses the RGG3 (549-638) region from the RNA-binding protein EWS
    Depositor
    David Libich
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-548
    Promoter
    T7
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238370-plasmid-map-sequence-id-478744

    Enlarge

  12. NanoLuc-TAF1(BD2) Fusion Vector

    Plasmid
    #237201
    Purpose
    Express NanoLuc(R)-TAF1(BD2) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    TAF1 (TAF1 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    BD2 domain only (residue 1515-1669)
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237201-plasmid-map-sequence-id-476793

    Enlarge

  13. EIF2AK2(252-551)-NanoLuc Fusion Vector

    Plasmid
    #237172
    Purpose
    Express EIF2AK2(252-551)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    EIF2AK2 (EIF2AK2 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Truncation of full-length protein; expressing res…
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237172-plasmid-map-sequence-id-476789

    Enlarge

  14. LgBiT-KRAS 4B(G12V) BiBiT-Ready Vector

    Plasmid
    #237076
    Purpose
    Express LgBiT-KRAS 4B(G12V) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237076-plasmid-map-sequence-id-471923

    Enlarge

  15. LgBiT-KRAS 4B G12C BiBiT Ready Vector

    Plasmid
    #237078
    Purpose
    Express LgBiT-KRAS 4B G12C Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237078-plasmid-map-sequence-id-471925

    Enlarge

  16. HiBiT-KRAS(G12C) Fusion Vector

    Plasmid
    #236889
    Purpose
    Express HiBiT-KRAS(G12C) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HiBit
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236889-plasmid-map-sequence-id-472710

    Enlarge

  17. NanoBRET Assay Vector, HaloTag-KRAS

    Plasmid
    #236871
    Purpose
    Express HaloTag(R)-KRAS in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236871-plasmid-map-sequence-id-473394

    Enlarge

  18. NanoBRET Assay Vector, NanoLuc-NRAS(Q61R)

    Plasmid
    #236863
    Purpose
    Express NanoLuc(R)-NRAS Q61R in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236863-plasmid-map-sequence-id-493429

    Enlarge

  19. NanoBRET Assay Vector, HaloTag-KRAS(G13D)

    Plasmid
    #236856
    Purpose
    Express HaloTag(R)-KRAS(G13D) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    G13D
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236856-plasmid-map-sequence-id-471852

    Enlarge

  20. NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)

    Plasmid
    #236854
    Purpose
    Express NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61K
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236854-plasmid-map-sequence-id-477412

    Enlarge

  21. SmBiT-KRAS4B(G12D) BiBiT-Ready Vector

    Plasmid
    #236798
    Purpose
    Express SmBiT-KRAS4B(G12D) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS4B(G12D) (KRAS Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236798-plasmid-map-sequence-id-473530

    Enlarge

  22. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

    Enlarge

  23. rTetR-GFP-p65

    Plasmid
    #232633
    Purpose
    p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition
    Depositor
    Karsten Rippe
    Insert
    rTetR-GFP-p65 (RELA Synthetic, Human)
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232633-plasmid-map-sequence-id-463626

    Enlarge

  24. pdCas9-GFP-p65

    Plasmid
    #232630
    Purpose
    p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer
    Depositor
    Karsten Rippe
    Insert
    dCas9-GFP-p65 (RELA Synthetic, Human)
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232630-plasmid-map-sequence-id-463624

    Enlarge

  25. Kif3A-EGFP-SNAP

    Plasmid
    #229852
    Purpose
    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif3A (Kif3a Mouse)
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229852-plasmid-map-sequence-id-460709

    Enlarge

  26. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

    Enlarge

  27. E547* FL USP7

    Plasmid
    #229001
    Purpose
    E547-mutant of human USP7
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-546 (USP7 Human)
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    T7
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229001-plasmid-map-sequence-id-458328

    Enlarge

  28. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid
    #223720
    Purpose
    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted start codon
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223720-plasmid-map-sequence-id-457431

    Enlarge

  29. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid
    #222015
    Purpose
    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    stop codon (TGA) is deleted
    Promoter
    chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222015-plasmid-map-sequence-id-457427

    Enlarge

  32. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

    Enlarge

  34. pWR23

    Plasmid
    #202770
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202770-plasmid-map-sequence-id-417278

    Enlarge

  35. pWR10

    Plasmid
    #202710
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202710-plasmid-map-sequence-id-417297

    Enlarge

  36. pWR9

    Plasmid
    #202645
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202645-plasmid-map-sequence-id-409735

    Enlarge

  40. SUMO CRYGS 16-177 Q16C

    Plasmid
    #217673
    Purpose
    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Tags
    6XHis and SUMO
    Expression
    Bacterial
    Mutation
    Glutamine 16 changed to Cysteine, and amino acids…
    Promoter
    T7
    Available Since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217673-plasmid-map-sequence-id-433254

    Enlarge

Showing: 8161 - 8200 of 9474 results