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Plasmid#187184PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32F, L33FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H
Plasmid#187185PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90A, V91APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H
Plasmid#187183PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32A, L33APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-Alox12-PLAT-EGFP-6H
Plasmid#187190PurposeProtein ExpressionDepositorInsertENSDARG00000069463 (alox12 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-hs-PKCgamma-C2-EGFP-6H
Plasmid#187115PurposeProtein ExpressionDepositorInsertENSG00000126583 (PRKCG Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0032
Plasmid#185623PurposeLevel 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCGDepositorInsertNbU6-1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H
Plasmid#187187PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationD34K, D87KPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H
Plasmid#187186PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90F, V91FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK_SEAP
Plasmid#154461PurposeExpression of SEAP and firefly luciferaseDepositorInsertSEAP
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB202
Plasmid#169113PurposeTESEC expression vector for mEGFPDepositorInsertmEGFP
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2351_Tier3(SB)-Zeo
Plasmid#169648PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)DepositorInsertPRPBSA-driven ZeoR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-SNAP-MD-OCT4
Plasmid#115691PurposeConstitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a mitotic degron (MD)DepositorInsertSNAP-MD-OCT4
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.N3-myc-SLC35A2
Plasmid#186285Purposetransient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cellsDepositorInsertSLC35A2 (SLC35A2 Human)
UseTagsMYCExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2403-Tier1-PhCMV-ZeoR
Plasmid#169495PurposeTier-1 vector encoding PhCMV-driven zeocin resistance gene ZeoR (PhCMV-ZeoR-pA).DepositorInsertPCMV-driven Sh ble
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Arkadia
Plasmid#180372Purposeexpressing mouse Arkadia proteinsDepositorInsertArkadia (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterLTR promoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1
Plasmid#183404PurposeDual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-GID-SmBiT-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
Plasmid#183399PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6A_L
Plasmid#146843PurposeInsect Expression of HsTNRC6ADepositorInsertHsTNRC6A (TNRC6A Human)
UseTagsExpressionInsectMutationA339T, K1578E compared to the sequence of isoform…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T
Plasmid#147580PurposeInsect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666DepositorInsertDm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PC(1-86)-H6
Plasmid#183780PurposeExpress PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
1046E=dgRNAs[traB,bTub]
Plasmid#149425PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.DepositorInsertU6.3-gRNAs[TraB, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterU6.3Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Scp20_18-49-GB1_AF
Plasmid#148720PurposeBacterial Expression of Scp20_18-49DepositorInsertScp20_18-49 (CAF20 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-ScEap1p_91-150_AF
Plasmid#148710PurposeBacterial Expression of ScEap1p_91-150DepositorInsertScEap1p_91-150 (EAP1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin_AD
Plasmid#148531PurposeMammalian Expression of DmRoquinDepositorInsertDmRoquin (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1200-1384_L
Plasmid#146825PurposeInsect Expression of DmGW182_1200-1384DepositorInsertDmGW182_1200-1384 (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SPSB1_SPRY
Plasmid#178487PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertSPSB1_SPRY (SPSB1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-510-V5His6_D
Plasmid#146151PurposeInsect Expression of Dm4E-T_1-510DepositorInsertDm4E-T_1-510 (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_1-440-V5His6_D
Plasmid#146123PurposeInsect Expression of DmEDC3_1-440DepositorInsertDmEDC3_1-440 (Edc3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T_1-510-V5His6_D
Plasmid#146149PurposeInsect Expression of Dm4E-T_1-510DepositorInsertDm4E-T_1-510 (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 Y635A
Plasmid#175772PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635ADepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationY635APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 N637A
Plasmid#175773PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637ADepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationN637APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_APV-SBP-Dendra2-ER-5
Plasmid#170679PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.DepositorInsertsAPV-SBP-Dendra2-ER-5
streptavidin-KDEL
UseTagsExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PB1-AG-FRB-TEVuncs-mTagBFP2-HA
Plasmid#178869PurposeMammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HADepositorInsertPB1-AzamiGreen-FRB-TEVuncs(NEYLQFL)-mTagBFP2-HA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry-Zdk1-Vav2cat
Plasmid#178875PurposeMammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1DepositorInsertmCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL95
Plasmid#168329PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi514802025
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL316
Plasmid#168377PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os08g40130
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL328
Plasmid#168389PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP48
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL360
Plasmid#168421PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os01g70240
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL373
Plasmid#168434PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP12
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL362
Plasmid#168423PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os04g32860
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL350
Plasmid#168411PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaCCH
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL386
Plasmid#168447PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP07
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL351
Plasmid#168412PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP31
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL372
Plasmid#168433PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP16
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL348
Plasmid#168409PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP45
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL361
Plasmid#168422PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP40
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL374
Plasmid#168435PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os02g48170
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL363
Plasmid#168424PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP10
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL375
Plasmid#168436PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP27
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL387
Plasmid#168448PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os04g39370
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only