We narrowed to 7,286 results for: 11

1. JPF0522

   Plasmid

   #124013

   Purpose

   Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-Rab4aM-7

   Plasmid

   #56441

   Purpose

   Localization: Ras GTPase, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

   Insert

   Rab4a (RAB4A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation removes NheI site

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pCDNA3 Flag MKK3b(Glu, delete LXL)

   Plasmid

   #50450

   Purpose

   activates p38 in the signaling pathway

   Depositor

   Roger Davis

   Article

   Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

   Insert

   MKK3b (MAP2K3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted L25-I27, changed Serine 218 to Glutamic A…

   Promoter

   CMV

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-ERKsub(TA)DD-Venus-NES

   Plasmid

   #84631

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ERKsub(TA)DD-Venus-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Mutated target Thr residue to Ala.

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. JPF0454

   Plasmid

   #124039

   Purpose

   Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pHR_pPGK1-spCAS9-P2A-mAzamiGreen
   

   Expression

   Mammalian

   Available Since

   Feb. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK8b_009

   Plasmid

   #123896

   Purpose

   Encodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   LA_hThal5.10-2-hg18
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK234_027

   Plasmid

   #123907

   Purpose

   Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA1 -hThal5.10-2_hg18
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. fgfprom luc

   Plasmid

   #69446

   Purpose

   basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Insert

   minimal fgf4 promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB3038(XII-1 MarkerFree)

   Plasmid

   #73278

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB3040(XII-4 MarkerFree)

    Plasmid

    #73280

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pClneoMyc' CST-1

    Plasmid

    #66859

    Purpose

    Express Myc-tagged C.elegans CST-1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

    Insert

    CST-1 (cst-1 Nematode)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S48N, H50R, S257P and V340A mutations in CST-1 co…

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MTK0_001

    Plasmid

    #123924

    Purpose

    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    TU-sgRNA - L1/RE
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLP-mEos2-Cep123

    Plasmid

    #72589

    Purpose

    mEos2 photoconvertible fluorescent protein fused to the N-terminus of isoform 1 of Cep123 (Cep89/CCDC123)

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Cytoskeleton (Hoboken). 2011 Nov;68(11):

    Insert

    Cep123 (CEP89 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK0_049

    Plasmid

    #123946

    Purpose

    Encodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hThal5.10-2_hg18 landing pad destination vector
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-AR-4-52

    Plasmid

    #104199

    Purpose

    Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

    Insert

    human androgen receptor aa 4-52 (AR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Flag-Trib2

    Plasmid

    #131155

    Purpose

    Mammalian expression of flag-tagged mouse TRIB2

    Depositor

    Warren Pear, Karen Keeshan

    Article

    Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

    Insert

    Trib2 (Trib2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB3037(XI-5 MarkerFree)

    Plasmid

    #73277

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTW088

    Plasmid

    #115936

    Purpose

    3'UTR insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 3'UTR No E1
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW094

    Plasmid

    #115937

    Purpose

    3'UTR insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 E1-3'UTR End
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AI-CN590

    Plasmid

    #62596

    Purpose

    KCTD13 N-terminus disruption by homolgy-directed repair

    Depositor

    Joshua Grimley

    Article

    Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

    Insert

    Cerulean-P2A-PuroR (KCTD13 Human)
    

    Use

    TALEN; Homology-directed repair donor

    Expression

    Mammalian

    Promoter

    hEF1alpha

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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