We narrowed to 20,285 results for: ACE;

1. pCEP4-FLAG-Tapasin

   Plasmid

   #141308

   Purpose

   Mammalian expression of FLAG-tagged Tapasin

   Depositor

   Erik Procko

   Article

   McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

   Insert

   TAPBP (TAPBP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-GFP11x7-HygR

   Plasmid

   #223676

   Purpose

   To check the split GFP signals in mitochondria (2)

   Depositor

   Juan Melero-Martin

   Article

   Lin et al Nature. 2024 May;629(8012):660-668. doi:

   Insert

   GFP11x7
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMG343

   Plasmid

   #176183

   Purpose

   preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   TPK2+ccdB dropout cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSS9

   Plasmid

   #71655

   Purpose

   Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli

   Depositor

   Ryan Gill

   Article

   Bassalo et al ACS Synth Biol. 2016 Apr 22.

   Insert

   gfpuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7A1

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nanog5P reporter

   Plasmid

   #16337

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

   Insert

   Nanog promoter (Nanog Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Nanog promoter

   Available Since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestattB/CY (pCM327)

   Plasmid

   #68341

   Purpose

   Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

   Depositor

   Leonard Zon, Christian Mosimann

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Type

   Empty backbone

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-mEGFP(A206K)-paCaMKII-ShadowG

   Plasmid

   #167475

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GZnP2 in pcDNA3.1+

   Plasmid

   #161739

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

   Insert

   GZnP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBMN HAYFP-LOV24

   Plasmid

   #49570

   Purpose

   regulate protein stability with light

   Depositor

   Thomas Wandless

   Article

   Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

   Insert

   YFP-LOV24
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   delete the last 3 aa from AsLOV2, with I532A muta…

   Promoter

   LTR

   Available Since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. vYM140_PB-pEFa1-iaaM(P.s)-Hygro

    Plasmid

    #160043

    Purpose

    Expressing iaaM for converting L-Trp to IAM

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Insert

    iaaM (iaaM )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC-ARS-CRISPR-v2

    Plasmid

    #99863

    Purpose

    Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. SadCas9 VPR

    Plasmid

    #188514

    Purpose

    Expresses FLAG tagged SadCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSAR3.5t

    Plasmid

    #210257

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    eBFP2
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

    Plasmid

    #165429

    Purpose

    Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    FHS-paCaMKII
    

    Use

    AAV

    Tags

    FHS(Flag, HisX6, Strep) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII0.4

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FLEX-SaCas9-U6-sgSlc17a8

    Plasmid

    #124848

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCKan-phoAp-GFPuv

    Plasmid

    #139072

    Purpose

    Low phosphate inducible promoter phoAp for GFP expression

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

    Insert

    phoAp-GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLP2

    Plasmid

    #209989

    Purpose

    Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Arf Q71L CFP

    Plasmid

    #128149

    Purpose

    Expression of Arf Q71L

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 2002 May 9;417(6885):187-93. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q71L

    Promoter

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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