We narrowed to 4,307 results for: ARA-2

1. pCMV MN (eGFP)

   Plasmid

   #89932

   Purpose

   Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   MN
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   M.SssI residues 2-272 fragment

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSiMPlh_N + pSiMPlh_C

   Plasmid

   #134316

   Purpose

   Maintenance of 2 plasmids with a single antibiotic in E. coli

   Depositor

   Barbara Di Ventura

   Article

   Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

   Insert

   hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   P254Q in hygR_gp41-1 N-intein

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEGFP-N1-SAV1

   Plasmid

   #166476

   Purpose

   Expresses fusion of mEGFP and SAV1

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, SAV1 (SAV1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Snap-Omp25

   Plasmid

   #69599

   Purpose

   Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

   Depositor

   David Sabatini

   Article

   Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

   Insert

   Snap-Omp25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJB02

   Plasmid

   #111959

   Purpose

   Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Inserts

   mEGFP
   eDHFR
   

   Use

   Tags

   DHFR

   Expression

   Bacterial

   Mutation

   eDHFR;residues 2-159 and enhanced GFP with monome…

   Promoter

   T7

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. ppBAalbNixE1E2 OpIE2-GFP

   Plasmid

   #173505

   Purpose

   transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

   Inserts

   Nix masculinisation gene
   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Nix isoforms with exons 1, 2 separated by intron 1

   Promoter

   OpIE2 and own

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-24p3/LCN2-282-LUC

   Plasmid

   #46865

   Purpose

   murine 24p3 minimal promoter as a luciferase reporter

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   24p3/lipocalin 2 proximal promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   24p3 minimal promoter

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET28a Gx-NL2

   Plasmid

   #169107

   Purpose

   Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-24p3/LCN2-145-LUC

   Plasmid

   #46866

   Purpose

   murine 24p3 minimal promoter as a luciferase reporter

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   24p3/lipocalin 2 proximal promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   24p3 promoter (inactive)

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pFB_ROSA26_SpCas9_gRNAs

    Plasmid

    #174703

    Purpose

    Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

    Depositor

    Beverly L. Davidson

    Article

    Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

    Insert

    SpCas9 dual gRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PLKO.1-Cd155 shRNA1

    Plasmid

    #166487

    Purpose

    Mouse Cd155 RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for mouse Cd155 (Pvr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-N1-MOB1

    Plasmid

    #166477

    Purpose

    Expresses fusion of mCherry and MOB1

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, MOB1 (MOB1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. ppBAalbNixE1E2 PUb-GFP

    Plasmid

    #173667

    Purpose

    transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Inserts

    Nix masculinisation gene
    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Nix isoforms with only exons 1, 2 separated by in…

    Promoter

    Aedes aegypti Polyubiquitin and cloned Nix promot…

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHisAlix deltaPRD

    Plasmid

    #42577

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pBEL2251

    Plasmid

    #170403

    Purpose

    For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    8xHis2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ700-717, replace with 225-249)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. retro-gfpIkkb-puro vector

    Plasmid

    #58251

    Purpose

    Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Ikkβ (Ikbkb Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO His6 SUMO SnRK2.6 (11-362)

    Plasmid

    #177847

    Purpose

    Bacterial Expression of SnRK2.6

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Use

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOGS539_rPD-L1nb

    Plasmid

    #226717

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag, Histidine tag, and Mating factor alpha se…

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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