-
Plasmid#195297PurposepACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).DepositorInsertTnsABC operon of McCAST (Tn7575)
UseTagsExpressionBacterialMutationPromoterIPTG inducible lac promoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10_K138/139R
Plasmid#127138DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK138R/K139RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDS12_mTurquoise2
Plasmid#136089PurposeFP for N-term fusion with a CTAGDepositorInsertCDS12_mTurquoise2(noStop)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDS_LUC
Plasmid#136083PurposeFirefly luciferaseDepositorInsertCDS_LUC
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb66
Plasmid#159422PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66DepositorInsertSb66
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWS1837
Plasmid#123058Purpose_-factor pheromoneDepositorInsert_-factor [3-4a]
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA
Plasmid#64983PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2_174
Plasmid#199106PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTK150
Plasmid#183126PurposeBTK Type 2 promoter part for golden gate assemblyDepositorInsertReverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
BPK2014-AmCas12b
Plasmid#121951PurposeE. coli expression, protein purificationDepositorInsertAmCas12b
UseTags10xHis tagExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3
Plasmid#106115PurposeLentiviral expression of PRKRA delta3DepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsDDK and mycExpressionMutationTruncationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCG-D-mSpyCatcher-His6-E
Plasmid#87376PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assDepositorInsertmSpyCatcher
UseSynthetic BiologyTagsHis6 tagExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046940
Plasmid#149261PurposeBacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL2_177
Plasmid#199109PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12
Plasmid#106116PurposeLentiviral expression of PRKRA delta 12DepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsExpressionMutationTruncationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pATD
Plasmid#141012PurposeMutisite Gateway mediated vector constructionDepositorInsertHSV-TK
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWS1037
Plasmid#123049PurposeG_ subunitDepositorInsertGpa1 [3-4a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB207
Plasmid#68598PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DSCP-Gateway
Plasmid#71507PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Apr)
Plasmid#110078PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only