We narrowed to 7,271 results for: Tet-on

1. pCAG-scFvGCN4sfGFP-VP64-GB1

   Plasmid

   #141417

   Purpose

   Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

   Depositor

   Izuho Hatada

   Article

   Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

   Insert

   scFvGCN4sfGFP-VP64-GB1
   

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Marathon-RT pentamutant for Split-PE (pEH107)

   Plasmid

   #190110

   Purpose

   Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, N26R, D74R, N116K, N197R

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBJ7

   Plasmid

   #167136

   Purpose

   A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Kanamycin promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLifeAct-mTurquoise2

   Plasmid

   #36201

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   LifeAct
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

   Plasmid

   #211469

   Purpose

   Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ATAD3A (ATAD3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

   Plasmid

   #211463

   Purpose

   Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI-dead
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D211A, E225A, K227A

   Promoter

   TRE

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-dCas9-5xPlat2AflD

   Plasmid

   #82560

   Purpose

   Expression vector for Cas9 peptide array (linker length: 22aa).

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   dCas9-5xPlat2AflD
   

   Expression

   Mammalian

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmTurquoise2-Peroxi

   Plasmid

   #36203

   Purpose

   In vivo visualization of the peroxisome (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   Peroxisomal targeting sequence
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-MBP-TEVpCS-ADGRE3 CTFdeltaTA-FLAG

   Plasmid

   #217724

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE3 (ADGRE3 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-344

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG

    Plasmid

    #217723

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE2 (ADGRE2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-523

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-Dlx-tTA

    Plasmid

    #210730

    Purpose

    AAV vector expressing tetracycline-controlled transactivator (tTA) under Dlx promoter (for expressing tTA in GABAergic neurons)

    Depositor

    Wei Xu

    Article

    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    tetracycline-controlled transactivator (tTA)
    

    Use

    AAV

    Promoter

    Dlx

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MBP-TEVpCS-ADGRE1 CTFdeltaTA-FLAG

    Plasmid

    #217722

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE1 (EMR1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-590

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-MBP-TEVpCS-ADGRF2 CTFdeltaTA-FLAG

    Plasmid

    #217728

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF2 (ADGRF2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-435

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHL 100

    Plasmid

    #52989

    Purpose

    pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    OxyS sRNA
    fhlA
    

    Use

    Synthetic Biology

    Tags

    GFP and RBS-mCherry

    Expression

    Bacterial

    Mutation

    −309 to +96 bp relative to start codon

    Promoter

    pLlac0-1 and pLtetO-1

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. lenti-CMV-TFAM-BioID2-IRES-Puro

    Plasmid

    #211465

    Purpose

    Lentivirus backbone expressing human TFAM fused with BioID2

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    TFAM-BioID2
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScalps_Puro

    Plasmid

    #99636

    Purpose

    Empty backbone, 3rd generation lentiviral vector

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Promoter

    SFFVp

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTP813

    Plasmid

    #113308

    Purpose

    human Mre11 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

    Insert

    Mre11 (MRE11 Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmTurquoise2-C1

    Plasmid

    #60560

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

    Plasmid

    #211468

    Purpose

    Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    4xGFP11-SEC61B
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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