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pSB700 lenti gRNA Puro_zhang2.0

Plasmid

#167919

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK1004 (pSL3)

Plasmid

#190999

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSW457 - tCLV3

Plasmid

#115980

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSW455 - pCLV3

Plasmid

#115979

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_C3012

Plasmid

#91099

Purpose

Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

PvUbi1

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn

Plasmid

#206965

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pVL26 - pSCR

Plasmid

#116021

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pSCR

Use

Unspecified

Mutation

T171A by SDM to abolish Eco31I site

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A0108

Plasmid

#91001

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMC-6-WDV-Rep-RepA-9

Plasmid

#197716

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

WDV Rep/RepA proteins: native ORFs

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-WDV-LS-L-11

Plasmid

#197719

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

WDV LSL in c-sense with GG expansion to v-sense ORF position

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-RedFLuc-9

Plasmid

#197738

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

RedF Luciferase (tomato optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-mVenus-I

Plasmid

#163763

Purpose

Expresses Streptomyces codon-optimised mVenus-I

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

mVenus-I

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

SP44

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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mc2 mCherry EF1a

Plasmid

#206225

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSW614 - pREV

Plasmid

#115989

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pREV

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAtCas9_3

Plasmid

#91158

Purpose

Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Expression

Plant

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A0901

Plasmid

#91025

Purpose

Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-AtCas9

Use

CRISPR

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPD1029 ZF43x6-Compact(CMVMin)_EYFP

Plasmid

#138913

Purpose

EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact(CMVmin)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact(CMVmin)

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTK333

Plasmid

#59574

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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