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LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

Plasmid

#170237

Purpose

Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJV132

Plasmid

#124254

Purpose

Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 3

Depositor

Stanley Riddell

Article

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Kras-Blasticidin

Plasmid

#120714

Purpose

Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

amino acids 158-188

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Plasmid

#159692

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Depositor

Dominic Esposito

Article

Procter et al Protein Expr Purif. 2018 Nov;151:99-105.

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Insect

Mutation

M188L

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLL5.0 mCherry_K-RasC14

Plasmid

#101286

Purpose

Lentiviral expression of K-Ras C14

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

K-Ras (KRAS Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

C14

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-0K9Q

Plasmid

#221743

Purpose

Stable expression of GFP-0K9Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-1K8Q

Plasmid

#221742

Purpose

Stable expression of GFP-1K8Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-3K6Q

Plasmid

#221741

Purpose

Stable expression of GFP-3K6Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRXIP-GFP-5K4Q

Plasmid

#221740

Purpose

Stable expression of GFP-5K4Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-HaloTag-KRas4B(CT)

Plasmid

#214276

Purpose

Mammalian expression of HaloTag fused to the C-terminal tail of KRas4B

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

HaloTag7-KRas4B(174-188) (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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