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pCMV-Sport6-CD9-pHluorin
Plasmid#130905PurposeExpression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD9-pHluorin (CD9 Aequorea victoria, Human)
UseTagspHluorinExpressionMammalianMutationphluorin inserted between Asn-50 and Asn-51 in ex…PromoterCMVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113611PurposePPI reporterDepositorInsertmu opioid receptor
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a his6-GST-WTPhi29DNAP
Plasmid#166863PurposeTo produce a dual affinity-tagged Phi29 DNA polymeraseDepositorInsertPhi29 Polymerase codon-optimized for overexpression in E. coli
UseTagsGST and his6ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-BroccoliSamSensor#1
Plasmid#124363PurposeOverexpression of circular RNA (Broccoli-based SAM sensor)DepositorInsertTornado-BroccoliSamSensor#1
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-Broccoli
Plasmid#66788PurposeExpresses Broccoli in the F30 scaffold in bacterial cellsDepositorInsertBroccoli in the F30 scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA
Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LacI
Plasmid#46394PurposeOverproduction of LacIDepositorInsertLacI
UseTags6xHIS tagExpressionBacterialMutationsilent T to C mutation at position 19Promoterarabinose promoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only
