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pLJM1 Flag GFP 4E-BP1 Omp25
Plasmid#112760Purposeexpress GFP-4EBP1-Omp25DepositorInsertFlag-GFP-4EBP1-Omp25 (EIF4EBP1 Human)
UseLentiviralTagsFlag eGFP and Omp25ExpressionMutationPromoterAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-DHFR-3xFLAG
Plasmid#217428PurposeLentiviral overexpression of human DHFRDepositorInsertDHFR (DHFR Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationInsert sequence is a codon-optimized gBLOCK (IDT)PromoterCMVAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgABCC4_1
Plasmid#217433PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4DepositorInsertsgRNA 1 targeting ABCC4 (ABCC4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgABCC4_3
Plasmid#217434PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4DepositorInsertsgRNA 3 targeting ABCC4 (ABCC4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
LMPd Amt LDHA
Plasmid#209408PurposeRetroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffoldDepositorInsertLdhA shRNA (Ldha Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307Y-pKK223
Plasmid#131381PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.DepositorInsertEcNGsC MutY chimera F307Y
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-pKK223
Plasmid#131382PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308V-pKK223
Plasmid#131393PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.DepositorInsertEcNGsC MutY chimera S308V
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308A-pKK223
Plasmid#131394PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera S308A
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS22H-IRE
Plasmid#133905PurposePositive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWHDepositorInsertIRE (Ftl1 Rat)
UseTagsExpressionYeastMutationPromoterPol IIIAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
nLuc-GLP-1R-His-FLAG
Plasmid#124831PurposeExpresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cellsDepositorInsertGLP1R (GLP1R Synthetic, Human)
UseLuciferaseTagsFLAG, His6, and NanoLucExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ALDOA_sgRNA1
Plasmid#201590PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertALDOA (ALDOA Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs
Plasmid#220493PurposeTo generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.DepositorInsertsgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-p53
Plasmid#133900PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorInsertp53 (Trp53 Mouse)
UseTagsExpressionYeastMutationPromoterT7Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-MIOS(1-875) in pRK5
Plasmid#184561PurposeCMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.DepositorInsertMIOS (MIOS Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBGE3.0
Plasmid#165988PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
neomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc-p53
Plasmid#133903PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorInsertp53 (TP53 Mouse)
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ALDOA_sgRNA2
Plasmid#201591PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertALDOA (ALDOA Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGE2.0
Plasmid#165984PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.DepositorInsertsRK2/RP4 origin of transfer
repA2B2C2
nourseothricin N-acetyl transferase
tetracycline resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only
