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pJK_ToeRepG2_N02_switch

Plasmid

#132742

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N02_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND2_L17_S19_S11

Plasmid

#132730

Purpose

T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND2_L17_S19_S11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND4_L17_S24_S11_S19_S13

Plasmid

#132732

Purpose

T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND4_L17_S24_S11_S19_S13

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAG_ToeRep_N09_switch

Plasmid

#132741

Purpose

T7 RNAP-driven expression of first-generation toehold repressor index 9 switch RNA with a GFP-ASV reporter

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRep_N09_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAG_Ptet*_ToeRep_N01_switch

Plasmid

#132737

Purpose

Inducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_ToeRep_N01_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N19_switch

Plasmid

#132744

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 19 switch RNA with a GFP-ASV reporter

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N19_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK_ToeRepG2_N64_switch

Plasmid

#132743

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporter

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N64_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_Ptet*_3WJrep_N19_switch

Plasmid

#132739

Purpose

Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_3WJrep_N19_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND3_L11_S11_S13_S19

Plasmid

#132731

Purpose

T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND3_L11_S11_S13_S19

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti 4.1 Ex miR200c-141

Plasmid

#35534

Purpose

3rd generation lentiviral vector

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

miR200c-141 genomic cluster (MIR200C, MIR141 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2012

Availability

Academic Institutions and Nonprofits only

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pSico

Plasmid

#11578

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pMIA10.7

Plasmid

#214451

Purpose

BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Inserts

Tet-On 3G
PuroR
TRE3G CopGFP

Use

Bxbi donor

Tags

PuroR, T2A, and Tet-On 3G

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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SGyA

Plasmid

#160292

Purpose

Drives expression of Cas9 and a TdTomato fluorescent marker

Depositor

Omar Akbari

Article

Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

Inserts

Vas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome

Use

Tags

T2A GFP

Expression

Insect

Mutation

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.8

Plasmid

#133521

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.16

Plasmid

#133536

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.-1

Plasmid

#133539

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.25

Plasmid

#133538

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.20

Plasmid

#133537

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.14

Plasmid

#133535

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.13

Plasmid

#133534

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge