We narrowed to 870 results for: Px330

1. pYFP-NEAT1pr_v2-RT

   Plasmid

   #97088

   Purpose

   Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   NEAT1pr_v2-RT (NEAT1 Human)
   

   Use

   CRISPR

   Tags

   EYFP

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PXL

   Plasmid

   #75349

   Purpose

   PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HeFm2SpCas9

   Plasmid

   #92111

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   “Highly enhanced Fidelity” mut2 SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

   Plasmid

   #173203

   Purpose

   Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)_No_FLAG_NPM1_G6

   Plasmid

   #178091

   Purpose

   Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9(1.1)_No_FLAG_LMNA_G2

   Plasmid

   #178090

   Purpose

   Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HeFSpCas9

   Plasmid

   #92355

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. HeFm1SpCas9

   Plasmid

   #92110

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRNF219.1.0-gDNA

   Plasmid

   #132464

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   RNF219
   

   Use

   CRISPR

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTACR TH1-p2aGFP

    Plasmid

    #135815

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Lee Rubin, Tim Ahfeldt

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 1 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTACR TH2-p2aGFP

    Plasmid

    #135816

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Lee Rubin, Tim Ahfeldt

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 2 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid

    #86610

    Purpose

    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid

    #86611

    Purpose

    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSOX11.1.0-gDNA

    Plasmid

    #176378

    Purpose

    Expresses spCas9 and gRNA sequence targeting SOX11

    Depositor

    Kevin White, Barbara Stranger

    Insert

    SOX11 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBACH2.2.0-gDNA

    Plasmid

    #176375

    Purpose

    Expresses spCas9 and gRNA sequence targeting BACH2

    Depositor

    Kevin White, Barbara Stranger

    Insert

    BACH2 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSOX2.1.0-gDNA

    Plasmid

    #176382

    Purpose

    Expresses spCas9 and gRNA sequence targeting SOX2

    Depositor

    Kevin White, Barbara Stranger

    Insert

    SOX2 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRERF1.1.0-gDNA

    Plasmid

    #176384

    Purpose

    Expresses spCas9 and gRNA sequence targeting TRERF1

    Depositor

    Kevin White, Barbara Stranger

    Insert

    TRERF1 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZNF627.1.0-gDNA

    Plasmid

    #176383

    Purpose

    Expresses spCas9 and gRNA sequence targeting ZNF627

    Depositor

    Kevin White, Barbara Stranger

    Insert

    ZNF627 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOSR1.1.0-gDNA

    Plasmid

    #176381

    Purpose

    Expresses spCas9 and gRNA sequence targeting OSR1

    Depositor

    Kevin White, Barbara Stranger

    Insert

    OSR1 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZNF608.2.0-gDNA

    Plasmid

    #176380

    Purpose

    Expresses spCas9 and gRNA sequence targeting ZNF608

    Depositor

    Kevin White, Barbara Stranger

    Insert

    ZNF608 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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