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pBiT3.1-N_KIF21A:1337-1653

Plasmid

#211213

Purpose

Mammalian expression of human KIF21A fragment L1337 to A1653. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

KIF21A:L1337-A1653 (KIF21A Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_NWD2:944-1662

Plasmid

#211319

Purpose

Mammalian expression of human NWD2 fragment S944 to S1662. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

NWD2:S944-S1662 (KIAA1239 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_NWD1:859-1462

Plasmid

#211295

Purpose

Mammalian expression of human NWD1 fragment P859 to V1462. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

NWD1:P859-V1462 (NWD1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

N926I natural variant (VAR_036773)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_KIF21A:1337-1653

Plasmid

#211287

Purpose

Mammalian expression of human KIF21A fragment L1337 to A1653. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

KIF21A:L1337-A1653 (KIF21A Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_EIF3B:186-685

Plasmid

#211279

Purpose

Mammalian expression of human EIF3B fragment V186 to T685. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF3B:V186-T685 (EIF3B Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_DENND3:894-1198

Plasmid

#211277

Purpose

Mammalian expression of human DENND3 fragment V894 to E1198. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DENND3:V894-E1198 (DENND3 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_WDR81:1619-1941

Plasmid

#211259

Purpose

Mammalian expression of human WDR81 fragment A1619 to A1941. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR81:A1619-A1941 (WDR81 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_NWD2:944-1662

Plasmid

#211245

Purpose

Mammalian expression of human NWD2 fragment S944 to S1662. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

NWD2:S944-S1662 (KIAA1239 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF1:1038-1400

Plasmid

#211052

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_DCAF1:1038-1400

Plasmid

#211053

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN155(I152L)-SRCR5

Plasmid

#207993

Purpose

BiFC assay

Depositor

Young Tang

Article

Huang et al Virol J. 2020 Jul 30;17(1):116. doi: 10.

Insert

SRCR5 (CD163 pig)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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6mSPL11F15R14::pBIB-BAS (6mSPL11)

Plasmid

#27387

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 mutant (AT1G27360 Mustard Weed)

Use

Used to create transgenic lines

Tags

Expression

Mutation

miR156 target sites of SPL11 mutated

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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SPL11F15R14::pBIB-BAS (gSPL11)

Plasmid

#27398

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 (AT1G27360 Mustard Weed)

Use

Used to create transgenic lines

Tags

Expression

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pSPL10F13R13::pBIB-BAS (gSPL10)

Plasmid

#27407

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 (AT1G27370 Mustard Weed)

Use

Used to create transgenic lines

Tags

Expression

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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6mpSPL10F13R13::pBIB-BAS (6mSPL10)

Plasmid

#27408

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 mutant (AT1G27370 Mustard Weed)

Use

Used to create transgenic lines

Tags

Expression

Mutation

miR156 target sites of SPL10 mutated

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-CMV1-mir144-CMV2-mir1

Plasmid

#164634

Purpose

Express miR-144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

mir144 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pbira(1-g78)-sec61b

Plasmid

#154455

Purpose

expresses flag-pbira(1-g78)-sec61b/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pbira(1-g78)-sec61b (SEC61B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

Plasmid

#169182

Purpose

Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp12-His6-3xFlag (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

His6-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBTG (pBigT-IRES-GFP; DM#268)

Plasmid

#15037

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Type

Empty backbone

Use

Cre/Lox; For making transgenic constructs

Tags

IRES-EGFP

Expression

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pBiex PKC - Peptide 8 GFP RFP

Plasmid

#83564

Purpose

Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

Insert

PKC alpha (PRKCA Human)

Use

Tags

10 nm ER/K Helix, FLAG purification tag, TEV Prot…

Expression

Bacterial and Insect

Mutation

Only catalytic domain of PKC (aa 335-672)

Promoter

T7 Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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