We narrowed to 593 results for: pFAST

1. Halo-ATG16L1 HRD

   Plasmid

   #207537

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Rev7-Halo HRD

   Plasmid

   #207079

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous REV7 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human REV7 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Halo-ATG2A HRD

   Plasmid

   #207540

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag flanked by human ATG2A locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Halo-MDC1 HRD

   Plasmid

   #207086

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous MDC1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag with internal PuroR cassette flanked by human MDC1 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Halo-ATM HRD

   Plasmid

   #207085

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag with internal PuroR cassette flanked by human ATM locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ATM-Halo HRD

   Plasmid

   #207082

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Halo-53BP1 HRD

   Plasmid

   #207087

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Halo-SHLD3 HRD

   Plasmid

   #207077

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD3 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag with internal PuroR cassette flanked by human SHLD3 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Halo-SHLD1 HRD

   Plasmid

   #207076

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag flanked by human SHLD1 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RcSxph

    Plasmid

    #194018

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Rif1-Halo HRD

    Plasmid

    #207081

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RNF169-Halo HRD

    Plasmid

    #207083

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Halo-SHLD2 HRD

    Plasmid

    #207078

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SNAP-LC3 HRD

    Plasmid

    #207545

    Purpose

    Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
    

    Use

    Tags

    SNAPtag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Halo-ATG5 HRD

    Plasmid

    #207542

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG5 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag flanked by human ATG5 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TR knockout HRD

    Plasmid

    #207579

    Purpose

    Homologous recombination donor replacing TR with a puro cassette.

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    puro resistance cassette flanked by TR locus sequences excluding TR
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Halo-LC3B HRD

    Plasmid

    #207539

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag with internal PuroR cassette flanked by human LC3B locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFB-MBD2.4Ch (pc2393)

    Plasmid

    #211730

    Purpose

    Expresses MBD2 (aa236-414) mCherry tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MBD2.4 aa236-414
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promotor

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFB-MBD2.3Ch (pc2392)

    Plasmid

    #211729

    Purpose

    Expresses MBD2 (aa222-414) mCherry tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MBD2.3 aa222-414
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promotor

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFB-MBD2.5R (pc2395)

    Plasmid

    #211727

    Purpose

    Expresses MBD2 (aa153-221) RFP tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MBD2.5 aa153-221
    

    Use

    Tags

    RFP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promotor

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge