We narrowed to 11,216 results for: SHA

1. pBLO 62.5

   Plasmid

   #123124

   Purpose

   Human expression vector for planctomyces CasX and sgRNA

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEJS887_pTetR-P2A-BFPnls/sgAlpha

   Plasmid

   #108651

   Purpose

   Alpha satellite repeats targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Alpha satellite repeats targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE2 GFP-Atl1

   Plasmid

   #86679

   Purpose

   Lentivirus to stably express fluorescent protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   ATL1 (ATL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTracer_Cr-TRP1

   Plasmid

   #64881

   Purpose

   Expresses CrTRP1 in mammalian cells

   Depositor

   Sebastián Brauchi

   Article

   Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

   Insert

   Transient receptor potential 1
   

   Use

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

5. qTAG-C-Solo-mStayGold-PXN

   Plasmid

   #227316

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold Tag (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHiUGE GS-gRNA (hSyn-Cre) mTubb3

   Plasmid

   #200369

   Purpose

   Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   Mouse Tubb3 gRNA and Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti_CamKIIa_mKate2

   Plasmid

   #96942

   Purpose

   To label mature excitatory iPSC-derived human neurons

   Depositor

   Ricardo Dolmetsch, Oleksandr Shcheglovitov

   Article

   Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

   Insert

   far-red fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E87D (please see depositor comments below)

   Promoter

   CaMKIIa

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX4T3-GST-EGFP

   Plasmid

   #177136

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSFDuet-1 IntS8 AA 1-308

   Plasmid

   #196905

   Purpose

   Expresses His-tagged Drosophila IntS8 amino acids 1-308

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 8 (IntS8 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST12.2 DYRK2 WT

    Plasmid

    #20005

    Depositor

    Anjana Rao

    Article

    Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

    Insert

    DYRK2 (DYRK2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

    Plasmid

    #176704

    Purpose

    neural activity dependent release of sbGLuc from presynaptic vesicles

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

    Inserts

    Gaussia luciferase, slow burn
    dTomato
    

    Use

    AAV

    Tags

    P2A and hPOMC1-26

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLXSN-Axl

    Plasmid

    #65222

    Purpose

    Expression of Axl in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

    Insert

    AXL (AXL Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

13. YFP-Rpp29-C1

    Plasmid

    #134545

    Purpose

    Expresses YFP-Rpp29 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Rpp29 (POP4 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-KRAS

    Plasmid

    #13919

    Depositor

    Frank Slack

    Article

    Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

    Insert

    KRAS 3'UTR (KRAS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Syn-FLEX-ExSYTE

    Plasmid

    #216263

    Purpose

    Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

    Depositor

    Susumu Tomita

    Article

    Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

    Insert

    ExSYTE P2A EGFP - Rat DTE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEVOL-AcKRS-CloDF

    Plasmid

    #127415

    Purpose

    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

    Plasmid

    #211687

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. shTCF7L2 # 1

    Plasmid

    #42557

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCCM

    Plasmid

    #162708

    Purpose

    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. THOC1

    Plasmid

    #156081

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    THOC1 (THOC1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. Nrxn3α-AS4-

    Plasmid

    #184332

    Purpose

    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAM4941 (pCV0053)

    Plasmid

    #120083

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3 Flag MKK4(glu)

    Plasmid

    #14813

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

    Insert

    MKK4(glu) (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-220Glu Thr-224Glu

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pBAD02_CBASS_Escherichia_coli_CVM_N37067PS

    Plasmid

    #224403

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli CVM N37067PS

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnF_AGS-C_SLATT6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD02_CBASS_Escherichia_coli_BIDMC103

    Plasmid

    #224402

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli BIDMC103

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnF_AGS-C_SLATT6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD02_CBASS_Escherichia_coli_K71-77

    Plasmid

    #224398

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli K71-77

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    Phospholipase_EcCdnE_DisA-like
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD02_CBASS_Escherichia_coli_RU1_BHI6

    Plasmid

    #224397

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    Cap15_EcCdnE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pL6-H2B-iRFP720-puro

    Plasmid

    #189992

    Purpose

    Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Human histone H2B
    

    Use

    Lentiviral

    Tags

    iRFP720

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge