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pFREDusk-KanR-MCS

Plasmid

#229735

Purpose

For far-red light induced bacterial expression of gene of interest

Depositor

Heikki Takala

Publication

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFREDusk-StrR-MCS

Plasmid

#229736

Purpose

For far-red light-induced bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Publication

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFREDusk-AmpR-MCS

Plasmid

#229737

Purpose

For far-red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Publication

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-MYO10 ΔF

Plasmid

#145816

Purpose

Expresses EGFP-tagged MYO10 with MyTH/FERM deletion in mammalian cells

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

Myo10 dFERM (MYO10 Human)

Tags

EGFP

Expression

Mammalian

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-Myo10 ΔF

Plasmid

#145139

Purpose

Expresses mScarlet-I tagged MYO10 construct with a MyTH/FERM domain deletion

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 ΔF (MYO10 Human)

Expression

Mammalian

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-FERM (Myo10)

Plasmid

#145140

Purpose

Expresses EGFP-tagged MyTH/FERM domains of MYO10.

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MyTH/FERM domains of MYO10 (MYO10 Human)

Expression

Mammalian

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-MYO10 TF

Plasmid

#145141

Purpose

Expresses EGFP-tagged MYO10 with MyTH/FERM swapped to Talin1 FERM domain.

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 TF (MYO10 Human)

Expression

Mammalian

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGB1-tail-mTurquoise2

Plasmid

#145142

Purpose

Shuttle vector with mTurquoise2-tagged ITGB1 cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGB1-tail-mTurquoise2 (ITGB1 Human)

Use

Gateway Cloning

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet

Plasmid

#145143

Purpose

Shuttle vector with YPet-tagged ITGA2 wild-type cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (ITGA2 Human)

Use

Gateway Cloning

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Publication

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Expression

Bacterial

Available since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TurboID-MEK1

Plasmid

#226169

Purpose

Used to transiently express a TurboID fusion of the protein kinase MEK1.

Depositor

Dustin Maly

Publication

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-TurboID-MEK1 (MAP2K1 Human)

Tags

Myc-TurboID

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Publication

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pNHA8

Plasmid

#223650

Purpose

BccP protein purification

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BccP

Tags

MBP

Expression

Bacterial

Promoter

LacI

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA10

Plasmid

#223554

Purpose

TurboID expression

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

TurboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCKC017

Plasmid

#226622

Purpose

pLenti wt hTdT template for libraries 1-2

Depositor

Chang Liu

Publication

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

Terminal deoxynucleotidyl transferase (DNTT Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

synonymous mutation in D2, synonymous mutation in…

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228i16

Plasmid

#209738

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[mCNPR43] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Publication

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228i24

Plasmid

#209739

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[pCNPR02] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Publication

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228q12

Plasmid

#209740

Purpose

ColA BBa_B0014 tetAp SD8 MjTyrRS-[p-TpaRS-1*] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Publication

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

MjTyrRS

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228r3

Plasmid

#209741

Purpose

ColA BBa_B0014 tetAp SD8 Lum1PylRS-[PylHRS*] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Publication

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

Mlum1RS

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228p

Plasmid

#209742

Purpose

ColA BBa_B0014 tetAp SD8 MmPylRS-[wt] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Publication

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

MmPylRS

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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