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NaVAe1 FL pIRES

Plasmid

#128076

Purpose

Mammalian cell expression vector for NaVAe1 bacterial sodium channel.

Depositor

Dan Minor

Publication

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NaVAe1

Expression

Mammalian

Available since

Dec. 24, 2020

Availability

Academic Institutions and Nonprofits only

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V954 - pDNR MCS SA no SA CmR - Donor

Plasmid

#11697

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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NaVSp1 FL pIRES

Plasmid

#128075

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel.

Depositor

Dan Minor

Publication

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NaVSp1

Expression

Mammalian

Available since

Dec. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylA

Plasmid

#48198

Purpose

Expresses CylA (fatty acyl-AMP ligase) in E. coli

Depositor

Emily Balskus

Publication

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylA

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pQO18 TEV-L400C

Plasmid

#27400

Depositor

Jonathan Javitch

Publication

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

HisX10 and TEV

Expression

Bacterial

Mutation

Changed Leu 400 to Cys

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D570A

Plasmid

#129673

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b-d570a entry …

Expression

Plant

Mutation

D570A; AacCas12b is rice codon optimized

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSADuet502

Plasmid

#46373

Depositor

Hideo Iwai

Publication

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaB intein

Tags

GB1 and H6-GB1

Expression

Bacterial

Promoter

T7

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pETO18G-H7C/R86C

Plasmid

#27403

Depositor

Jonathan Javitch

Publication

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

HisX8 and Thrombin

Expression

Bacterial

Mutation

Changes His 7 to Cys and Arg 86 to Cys

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pHelA12

Plasmid

#176493

Purpose

Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

Depositor

Xiaodong Cheng, Jian-Dong Huang

Publication

Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

Insert

SARS-CoV NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

V570I

Promoter

T7

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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NaVSp1 DeltaCC pIRES

Plasmid

#128084

Purpose

Mammalian cell expression vector for NaVSp1 1-236 chimera bacterial sodium channel.

Depositor

Dan Minor

Publication

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NaVSp1

Expression

Mammalian

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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HA tag NaVSp1 FL pIRES

Plasmid

#128087

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel. N-terminal HA tag.

Depositor

Dan Minor

Publication

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NaVSp1

Tags

HA

Expression

Mammalian

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-tglB

Plasmid

#134676

Purpose

Expresses His6-TglBin E. Coli

Depositor

Wilfred van der Donk

Publication

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglB

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1_p pET24b

Plasmid

#127852

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Publication

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Tags

3C Cleavage site, His Tag, and MBP

Expression

Bacterial

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB-puro-mU6-CNPshRNA2

Plasmid

#126612

Purpose

Expresses shRNA against human CNP under mouse U6 promoter

Depositor

Xiaozhong Wang

Publication

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP ShRNA-2

Use

RNAi

Promoter

Mouse U6 promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-chCARex

Plasmid

#87233

Purpose

bacterial expression of GST-CAR extracellular domain

Depositor

Fritz Rathjen

Publication

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pVRa27_4265

Plasmid

#49682

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF27_4265

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A-SRDX

Plasmid

#129684

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b-SRDX

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pP[RS3]3'M

Plasmid

#53552

Purpose

for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila

Depositor

Renjie Jiao

Publication

Yu et al Biol Open. 2014 Apr 15;3(4):271-80. doi:

Insert

AscI and MluI restriction enzyme sites

Expression

Insect

Promoter

same pP[RS3]

Available since

Dec. 3, 2014

Availability

Academic Institutions and Nonprofits only
p414GPD-3xFLAG-Nop4 WT

Plasmid

#169261

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 WT (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6_Ptrc_BsMdh_BmHps_BmPhi

Plasmid

#129104

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

Depositor

Eleftherios Terry Papoutsakis

Publication

Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

Insert

BsMDH BmHPS BmPHI

Expression

Bacterial

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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