We narrowed to 11,646 results for: emb

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pME EFS-rtTA/rtTA3 (JDW 1230)

Plasmid

#224525

Purpose

A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS-rtTA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CLC-EGFP

Plasmid

#193566

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) WT

Plasmid

#134695

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(G137I)

Plasmid

#133274

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Publication

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G137I

Promoter

CMVd2

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pmcsg7-mCRT(deltaC)

Plasmid

#83506

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Publication

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Mutation

delta C. Contains residues 1-339

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pRenilla (GB0056)

Plasmid

#68198

Purpose

Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla coding region

Use

Luciferase and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCMP-APr

Plasmid

#61184

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtPT4

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TGN61r

Plasmid

#61175

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP61

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-PDESr

Plasmid

#61207

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NET33 pmRFP-N2 (448)

Plasmid

#61979

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET33 (SCARA5 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
p5E-dr-myl7-pro (JDW 1148)

Plasmid

#229836

Purpose

A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

cmlc2/myl7

Use

Gateway Cloning

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

Plasmid

#224500

Purpose

Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mNeonGreen-HA-pA

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xNLS-mTagBFP2-Cre (JDW 918)

Plasmid

#224517

Purpose

A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xNLS-mTagBFP2-Cre

Use

Cre/Lox

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV SPOP 1-374

Plasmid

#197015

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Publication

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG3_nat

Plasmid

#184918

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pECFP-histone H3

Plasmid

#179698

Purpose

visualization of nucleus/chromosome

Depositor

Kenji Sugimoto

Publication

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Histone H3 (H3c10 Mouse)

Tags

ECFP

Expression

Mammalian

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTCF176 HLA-A*02:01 (CD8 mut)

Plasmid

#180459

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

HLA-A*02:01 (CD8 mut)

Tags

BSP41

Expression

Bacterial

Mutation

D227K, T228A, replacement of transmembrane domain…

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-GCP2

Plasmid

#178068

Purpose

Insect cell expression of GCP2

Depositor

Tarun Kapoor

Publication

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP2 (TUBGCP2 Human)

Expression

Insect

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pB-TAG-HES7

Plasmid

#130935

Purpose

PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Publication

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Expression

Mammalian

Promoter

TetO

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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PB-Haunt E1-PuroT2AmCherry-4A KI

Plasmid

#92164

Purpose

For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

Depositor

Xiaohua Shen

Publication

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt P-Inr2-cDNA KI homology arms

Use

Other

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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