We narrowed to 2,485 results for: organization Addgene

1. pLenti-VQRRA-P2A-GFP-PGK-Puro

   Plasmid

   #110864

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-VRERRA-P2A-GFP-PGK-Puro

   Plasmid

   #110865

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204191

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of R162

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

   Plasmid

   #204213

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Point mutation on I207M

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204211

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Point mutation on Q175* (*termination)

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204210

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Point mutation on R178* (*termination)

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

   Plasmid

   #204212

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NK2 homeobox 1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Point mutation on I207F

   Expression

   Mammalian

   Mutation

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b MOSPD2 [315-445]

   Plasmid

   #186474

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [315-445]

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.1 mitoAURKA Lys162Met

   Plasmid

   #157752

   Purpose

   Expression of kinase-dead AURKA K62M in mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP N1 AURKA 1-110

    Plasmid

    #157755

    Purpose

    Expression of AURKA Nter (aa1-110):GFP in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The first 110 amino acids of AURKA were cloned

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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