We narrowed to 21,628 results for: ESC

1. pCNL-C1_PTS1

   Plasmid

   #65700

   Purpose

   Expresses CNL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pONL-C1_PTS1

   Plasmid

   #65701

   Purpose

   Expresses ONL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pONL-N1_Mito

   Plasmid

   #65671

   Purpose

   Expresses Mito-ONL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   cytochrome c oxidase subunit VIII
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7 cyclin A1-164 venus cks1 (2196)

   Plasmid

   #61839

   Purpose

   expression of N terminal cyclin A and cks1

   Depositor

   Jonathon Pines

   Article

   Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

   Insert

   cyclin A (CCNA2 Human)
   

   Use

   In vitro transcription

   Tags

   Venus

   Expression

   Mutation

   L2V

   Promoter

   T7

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

5. mIFP12-CAF1-C-10

   Plasmid

   #56246

   Purpose

   Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CAF1
   

   Use

   Tags

   mIFP12

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. mIFP24-CAF1-C-10

   Plasmid

   #56266

   Purpose

   Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CAF1
   

   Use

   Tags

   mIFP24

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCVD419

   Plasmid

   #63754

   Purpose

   3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.

   Depositor

   James Kaper

   Article

   Levine et al J Infect Dis. 1987 Jul;156(1):175-82.

   Insert

   ehxA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHL 1410

   Plasmid

   #53011

   Purpose

   pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   DsrA sRNA
   ompC
   rpoS
   

   Use

   Synthetic Biology

   Tags

   T7noRBS (900bp) and gfp

   Expression

   Bacterial

   Mutation

   contains −150 to +30 bp relative to start codon a…

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. tdEos-Cx43-7

   Plasmid

   #57603

   Purpose

   Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Cx43
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pNB693

    Plasmid

    #60145

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    YeLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mPapaya-Rab4a-7

    Plasmid

    #56645

    Purpose

    Localization: Ras GTPase, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mPapaya

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHL1853

    Plasmid

    #53040

    Purpose

    PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    tetR
    csrA
    

    Use

    Synthetic Biology

    Tags

    LVA, RBS(st2), RBS(st3), and glgC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind and pLlac0-1

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pHL 990

    Plasmid

    #52992

    Purpose

    pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    DsrA sRNA
    RhyB sRNA (competitor)
    rpoS
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    includes −150 to +30 bp relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pHL 1022

    Plasmid

    #52996

    Purpose

    pLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    OxyS sRNA
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pHL 762

    Plasmid

    #52990

    Purpose

    pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    MicC sRNA
    sodB
    ompC
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    −56 to +141 bp and −81 to +36 bp

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pHL 1020

    Plasmid

    #52995

    Purpose

    pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    DsrA sRNA (competitor)
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pHL 1019

    Plasmid

    #52994

    Purpose

    pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    MicC sRNA (Competitor)
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pHL 1039

    Plasmid

    #53000

    Purpose

    pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    DsrA sRNA
    OxyS sRNA (competitor)
    rpoS
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains −150 to +30 bp relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pHL 1073

    Plasmid

    #53003

    Purpose

    pCon:ompC::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    mCherry
    GFP
    

    Use

    Synthetic Biology

    Tags

    ompC (target mRNA) and sodB (target mRNA)

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon a…

    Promoter

    pCon and pLlacO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pHL 1303

    Plasmid

    #53008

    Purpose

    pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    sodB
    ompC
    

    Use

    Synthetic Biology

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    contains -56 to +141 relative to start codon and …

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pPK1E-2

    Plasmid

    #48146

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPK1E-1

    Plasmid

    #48145

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHL1530

    Plasmid

    #37569

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    TetR
    CsrA
    

    Use

    Synthetic Biology

    Tags

    glgC leader region

    Expression

    Bacterial

    Mutation

    start codon removed

    Promoter

    pConNoHind and pLlacO-1

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pHL1355

    Plasmid

    #37566

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    TetR
    CsrA
    

    Use

    Synthetic Biology

    Tags

    glgC leader region

    Expression

    Bacterial

    Mutation

    start codon removed

    Promoter

    pConNoHind and pConNoHindM12

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

25. EC.SurA.pTYB1.a21-281&390-428.M231R

    Plasmid

    #15958

    Depositor

    David McKay

    Article

    Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

    Insert

    Escherichia coli surA delta PPIase domain 2
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    residues 282-389 deleted; M231R mutation

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3-PKAsub-YPet-Kras

    Plasmid

    #138206

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PIK3CA - Exon20 H1047R

    Plasmid

    #16639

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lyn FAK biosensor

    Plasmid

    #78299

    Purpose

    FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Lyn Fak biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KRas FAK biosensor

    Plasmid

    #78301

    Purpose

    FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    KRas FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA-R-iLACCO1.2

    Plasmid

    #208027

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA-HA-eLACCO2.1-NGR

    Plasmid

    #208023

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-R-iLACCO1

    Plasmid

    #208025

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA-R-iLACCO1.1

    Plasmid

    #208026

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEB2-mCherry2-L

    Plasmid

    #104003

    Purpose

    Plasmid encoding mCherry2-L

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCherry2-L
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGEE NNLA IIKEF… V…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pmTurquoise2-T2A-Venus(L68V)

    Plasmid

    #60494

    Purpose

    Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2-T2A-mVenus(L68V)
    

    Use

    Tags

    T2A peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR4_G3BP1

    Plasmid

    #127104

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHRm-NLS-dCas9-GFP11x7-NLS

    Plasmid

    #70224

    Purpose

    Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    NLS-dCas9-GFP11x7-NLS
    

    Use

    Tags

    GFP11x7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEvol-MjaYRS

    Plasmid

    #153557

    Purpose

    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

    Depositor

    Huiwang Ai

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

    Inserts

    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS and pBAD

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-HiBiT-CD9

    Plasmid

    #162592

    Purpose

    N-terminally HiBiT-tagged human CD9 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD9 (CD9 Human, Synthetic, human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pk7-GR-GFP

    Plasmid

    #15534

    Depositor

    Ian Macara

    Article

    Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The glucocorticoid receptor was cloned into the X…

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCDH_Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65267

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. AAVS1-Puro CAG FUCCI

    Plasmid

    #136934

    Purpose

    donor plasmid for constitutive FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    

    Use

    Synthetic Biology

    Tags

    Clover and mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. pXL001-TQM IL13 CAR

    Plasmid

    #154054

    Purpose

    Lentiviral expression of TQM IL13 CAR for CAR-T therapy

    Depositor

    Xiaojun Lian

    Article

    Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

    Insert

    TQM IL13 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. p189

    Plasmid

    #65207

    Purpose

    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pmScarlet-i_H2A_C1

    Plasmid

    #85053

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pTRIP-CMV-GFP-FLAG-cGAS

    Plasmid

    #86675

    Purpose

    Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pC1-SypHer3s-dmito

    Plasmid

    #108119

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-dmito
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCAG-G-Flamp1-mut

    Plasmid

    #188568

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pC1-SypHer3s-IMS

    Plasmid

    #108120

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-IMS
    

    Use

    Tags

    ratiometricYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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