We narrowed to 22,948 results for: Tes
-
Plasmid#235619PurposeEncodes ALIBi C-terminal fragment, residues 100-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequenceDepositorInsertALIBi C-terminal fragment
TagsNES-V5-eMagAFExpressionMammalianMutationresidues 100-321Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_eMagAF_C257
Plasmid#235631PurposeEncodes ALIBi C-terminal fragment, residues 257-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequenceDepositorInsertALIBi C-terminal fragment
TagsNES-V5-eMagAFExpressionMammalianMutationresidues 257-321Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_GFP_FTA
Plasmid#235633PurposeEncodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.DepositorInsertGFP
TagsFLAG-TEV cleavage site-AviTag and NES-V5ExpressionMammalianAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_eMagAF_C78
Plasmid#235617PurposeEncodes ALIBi C-terminal fragment, residues 78-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequenceDepositorInsertALIBi C-terminal fragment
TagsNES-V5-eMagAFExpressionMammalianMutationresidues 78-321Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_eMagAF_C74
Plasmid#235615PurposeEncodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequenceDepositorInsertALIBi C-terminal fragment
TagsNES-V5-eMagAFExpressionMammalianMutationresidues 74-321Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_eMagBF_N73
Plasmid#235635PurposeEncodes fusion protein of nuclear export sequence, V5, eMagBF (enhanced Magnet, basic, fast), ALIBi N-terminal fragment (residues 1-73) (order reversed)DepositorInsertALIBi N-terminal fragment
TagsNES-V5-eMagBFExpressionMammalianMutationresidues 1-73Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N167_eMagBF_V5_NES
Plasmid#235622PurposeEncodes ALIBi N-terminal fragment, residues 1-167, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
TagseMagBF-V5-NESExpressionMammalianMutationresidues 1-167Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N195_eMagBF_V5_NES
Plasmid#235624PurposeEncodes ALIBi N-terminal fragment, residues 1-195, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
TagseMagBF-V5-NESExpressionMammalianMutationresidues 1-195Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoRHA
Plasmid#232994PurposeGalactose iduced expression of Gcn4 SATtoG KtoRHAin yeastDepositorInsertGcn4 SATtoG KtoR
Tags3xHA and TEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoR
Plasmid#232961PurposeGalactose iduced expression of Gcn4 ATtoG KtoR in yeastDepositorInsertGcn4 SATtoG KtoR
TagsTEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoRGFP
Plasmid#233006PurposeGalactose iduced expression of Gcn4 SATtoG KtoRGFP in yeastDepositorInsertGcn4 SATtoG KtoR
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol W
Plasmid#232977PurposeGalactose iduced expression of Gcn4 solvvol W+ in yeastDepositorInsertGcn4 solvvol W+
Tags3xHA and TEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hRPL3-FLAG_WT
Plasmid#233616PurposeExpression of Flag-tagged hRPL3DepositorAvailable SinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hRPL3-FLAG_H245A
Plasmid#233617PurposeExpression of Flag-tagged hRPL3 with H245A mutationDepositorInsertFlag-tagged hRPL3 with H245A mutation (RPL3 Human)
TagsFlagExpressionMammalianMutationH245AAvailable SinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 SATtoG KtoR
Plasmid#231861PurposeBacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoRDepositorInsertGcn4 SATtoG KtoR
Tags6xHis, TEV cleavage siteExpressionBacterialMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterT7Available SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Monbr-Sox-like
Plasmid#231530PurposeExpresses Monsiga brevicolis full-length Sox-like in mammalian cells, for lentivirus generationDepositorInsertSox-like
UseLentiviralAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Salhel-I-K57E
Plasmid#231519PurposeExpresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generationDepositorInsertSalhel-I HMG K57E flanked with N- and C-termini of mSox2
UseLentiviralMutationPoint mutation at position 57 K to E of the HMGAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Salro-Sox-like
Plasmid#231531PurposeExpresses Salpingoeca rosetta full-length Sox-like in mammalian cells, for lentivirus generationDepositorInsertSox-like
UseLentiviralAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mNgn2-x3HA
Plasmid#233189PurposeRetroviral expression of tagged mNgn2x3HADepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mNgn2-x3FLAG
Plasmid#233190PurposeRetroviral expression of tagged mNgn2x3FLAGDepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mNgn2-x1HA
Plasmid#233191PurposeRetroviral expression of tagged mNgn2x1HADepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINF AtMKK5
Plasmid#228396PurposeRecombinant expression of His6-tagged Arabidopsis thaliana MKK5 in E. coliDepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
tdiRFP-BFP reporter
Plasmid#190184PurposeThe cre-activatable fluorescence reporterDepositorInsertstdiRFP
TagBFP2
ExpressionBacterial and MammalianPromoterCMVAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP-p65
Plasmid#232630Purposep65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacerDepositorInsertdCas9-GFP-p65 (RELA Synthetic, Human)
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BmNG
Plasmid#232204PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BmSc
Plasmid#232205PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 HopU1
Plasmid#228395PurposepENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tagsDepositorInsertHopU1
UseGateway-compatible entry vectorMutationAlanine inserted after start Methionine due to Nc…Available SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BmSc
Plasmid#232208PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTyDepositorInsertmScarlet
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmSc
Plasmid#232548PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
ExpressionBacterialMutationNoneAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 ILVtoED solvvol
Plasmid#231858PurposeBacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvolDepositorInsertGcn4 ILVtoED solvvol
Tags6xHis, TEV cleavage siteExpressionBacterialMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterT7Available SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7CNW)RS
Plasmid#222874PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
CamKII-mtPyronicSF
Plasmid#228906PurposeExpression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834DepositorInsertpyronic SF
UseAAVTagsCox8 targettingExpressionMammalianPromoterCamKIIAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE
Plasmid#225353PurposeLentiviral vector for DKK1 and BFP expressionDepositorAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
aTY50_pPGK1_Pcad_IRES_PuroR_WPRE
Plasmid#225348PurposeLentiviral vector for Pcad expressionDepositorAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol1 - 14xUAS:CaSR-EGFP
Plasmid#191502PurposeA UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.DepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol1 transgenes…Promoter14X UASAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) D221N E244Q
Plasmid#192053PurposeExpresses a 2X catalytic mutant of (ORF37) D221N E244QDepositorInsertSOX (D221N E244Q)
UseUnspecifiedMutation2X catalytic mutant D221N E244QPromoterCMVAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS2075
Plasmid#158098PurposeHigh Copy number plasmid containing smCBA insert.DepositorInsertsmCBA
PromotersmCBAAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
px552-sg-gria1-HT
Plasmid#187652PurposeContains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.DepositorInsertsHaloTag donor sequence
miRFP670
UseAAV and CRISPRTagsN/AExpressionMammalianPromoterSynapsinAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-WHG cGAS-HA
Plasmid#186867PurposeDoxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertWhite-handed gibbon cGAS with C-terminal HA tag
UseRetroviralTagsHAPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-EBFP2-FRB
Plasmid#222417PurposeTo express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)
Plasmid#225438PurposeTranscriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.DepositorInsertCBS-FRT-OCS-BeYDVRepRepA-tNos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)
Plasmid#225430PurposeOCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-FRT-OCSterm-FRT-miniDFR
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)
Plasmid#225431PurposeOCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-att-OCSterm-att-miniDFR
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2C
Plasmid#225070PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2C)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8589
Plasmid#175221PurposeXenopus oocyte expression vector containing YMR253C. Contains T7 RNA polymerase binding site, and adds polyA tail to YMR253C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYMR253C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8707
Plasmid#175222PurposeXenopus oocyte expression vector containing YNL095C. Contains T7 RNA polymerase binding site, and adds polyA tail to YNL095C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYNL095C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8728
Plasmid#175223PurposeXenopus oocyte expression vector containing YOL060C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL060C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOL060C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8711
Plasmid#175225PurposeXenopus oocyte expression vector containing YOR092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR092W
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8713
Plasmid#175226PurposeXenopus oocyte expression vector containing YOR271C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR271C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR271C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8590
Plasmid#175227PurposeXenopus oocyte expression vector containing YOR307C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR307C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR307C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only