We narrowed to 10,984 results for: Vars;

1. pXJ-Myc-GATA4

   Plasmid

   #232647

   Purpose

   transient expresses GATA4 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pV1524

   Plasmid

   #111431

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Equalizer-L episome

   Plasmid

   #169738

   Purpose

   Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCPH2A.X-GS3

   Plasmid

   #204745

   Purpose

   Expression of Cas9 and human H2AX

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. dxCas(3.7)-VPR

   Plasmid

   #108383

   Purpose

   Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   dxCas(3.7)-VPR
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDV06.2c_DART-VADAR_iRFP720-sensor

   Plasmid

   #200410

   Purpose

   pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Closed-loop DART VADAR sensor detecting iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-SpCas9(H840A)-T2A-EGFP

   Plasmid

   #221231

   Purpose

   Express nCas9 nickase (H840A) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(H840A)-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVET-IRES-GFP

   Plasmid

   #107139

   Purpose

   A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

   Depositor

   Aki Manninen

   Article

   Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BE4max-NRRH

   Plasmid

   #136918

   Purpose

   Expresses BE4max-NRRH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   BE4max-NRRH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-AID-BLM-UGI

    Plasmid

    #221218

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT/TO_TOMM20_HaloTag9

    Plasmid

    #169335

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BE4max-NRCH

    Plasmid

    #136920

    Purpose

    Expresses BE4max-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    BE4max-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-BE4max-NRTH

    Plasmid

    #136919

    Purpose

    Expresses BE4max-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    BE4max-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIP-SFFV-EGFP-NLS

    Plasmid

    #86677

    Purpose

    Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    EGFP-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-mRIN2

    Plasmid

    #108940

    Purpose

    Mammalian expression of mRIN2

    Depositor

    Guido Serini

    Article

    Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

    Insert

    Ras and Rab interactor 2 (Rin2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4_10xUAS-MLPmin-luc2

    Plasmid

    #194383

    Purpose

    Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    10xUAS-luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MLP minimal

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)

    Plasmid

    #138555

    Purpose

    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. hWIP1 FLAG

    Plasmid

    #28105

    Depositor

    Lawrence Donehower

    Article

    Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

    Insert

    WIP1 (PPM1D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_TXK-1/1

    Plasmid

    #91539

    Purpose

    Protein expression and purification of human SH3 domain construct TXK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TXK-1/1 (TXK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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