We narrowed to 10,321 results for: plasmids 101

1. pRF TpC hTERT intron-1

   Plasmid

   #171070

   Purpose

   Firefly-Renilla luciferase assay for hTERT promoter activity of hTERT intron-1

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   hTERT intron-1
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD_IPF2.0

   Plasmid

   #163124

   Purpose

   A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   IFP2.0
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-AP.Baz1a.1-GFP-WPRE

   Plasmid

   #211490

   Purpose

   expresses GFP under artificial promoter Baz1a

   Depositor

   Georg Keller

   Article

   O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgDonor_lentiGuide

   Plasmid

   #184168

   Purpose

   LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Insert

   sgDonor
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4 gRNA concatemer

   Plasmid

   #84881

   Purpose

   Empty concatmer vector in which 4 gRNAs can be inserted

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-AP.Agmat.1-GFP-WPRE

   Plasmid

   #211489

   Purpose

   expresses GFP under artificial promoter Agmat

   Depositor

   Georg Keller

   Article

   O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUB_FLuc100---MS2

   Plasmid

   #210574

   Purpose

   Expresses Optimal Firefly with 3'UTR MS2 hairpins

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Optimal Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2 gRNA concatmer

   Plasmid

   #84879

   Purpose

   Empty concatmer vector in which 2 gRNAs can be inserted

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

9. GEARBOCS-SPARCL1-N-HATag

   Plasmid

   #218185

   Purpose

   To tag Hevin with HA at the N-terminal

   Depositor

   Cagla Eroglu

   Article

   Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

   Insert

   sgRNA (Sparcl1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GEARBOCS-SPARCL1-C-mCherryTag

    Plasmid

    #218183

    Purpose

    To tag Hevin with mCherry at its C-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGb_PMLlxP_EGFP

    Plasmid

    #140283

    Purpose

    a targeting plasmid for EGFP-PML

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    EGFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    endogenous

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFP

    Plasmid

    #175593

    Purpose

    Lac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    CFP (cerulean)
    

    Use

    Tags

    Expression

    Mutation

    An improved monomeric CFP. See doi: 10.1038/nbt945

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3 gRNA concatemer

    Plasmid

    #84880

    Purpose

    Empty concatmer vector in which 3 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pJY3919

    Plasmid

    #113332

    Purpose

    Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRGEB34

    Plasmid

    #158152

    Purpose

    Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

    Plasmid

    #211491

    Purpose

    conditional expression of GCaMP8s under artificial promoter Adamts2

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    DIO-GCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

    Plasmid

    #211493

    Purpose

    conditional expression of GCaMP8s under artificial promoter Baz1a

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    DIO-GCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDB026e

    Plasmid

    #109261

    Purpose

    Accessory plasmid for PACE of MjTyrRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp_pIII(P29*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codon at position 29 and 184 of gIII

    Promoter

    Ppsp and PproK

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

    Plasmid

    #183077

    Purpose

    Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and crRNA ending with a HDV ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNeurog2-Luciferase reporter

    Plasmid

    #64126

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    Neurog2 promoter
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Neurog2

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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