We narrowed to 9,122 results for: Pol

1. ptrc99a-pVhb-tdTER-fdh

   Plasmid

   #215649

   Purpose

   pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   trans-enoyl-CoA reductase
   formate dehydrogenase
   

   Expression

   Bacterial

   Promoter

   Vhb

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. psicheck-2-Acsm2

   Plasmid

   #48158

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of Acsm2 (Acsm2 Mouse)
   

   Use

   Luciferase

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ABKAR(T/A)

   Plasmid

   #140052

   Purpose

   Negative control mutant for ABKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR(T/A)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC-4CL1

   Plasmid

   #35947

   Depositor

   Claudia Schmidt-dannert

   Article

   Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

   Insert

   4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLac-mod

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMT-PT2A-iRFP670-Tdtomato-GFP

   Plasmid

   #111817

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   Tdtomato
   GFP
   

   Use

   Cloning intermediate

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPM1001

   Plasmid

   #167548

   Purpose

   Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

   Depositor

   Renato Morona

   Article

   Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

   Insert

   Vibrio cholerae O antigens genes of the Inaba serotype
   

   Use

   Cosmid

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

8. GFAP-Lyn-mCherry-pHluorin

   Plasmid

   #187980

   Purpose

   Membrane targeted cytoplasmic fluorescent pH sensor

   Depositor

   Chris Dulla

   Article

   Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

   Insert

   Lyn-mcherry-pHluorin
   

   Use

   AAV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRB-ECFP(W66A)-Giantin

   Plasmid

   #67903

   Purpose

   Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   FRB-ECFP(W66A)-Giantin
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-UbC-mCherry Rab5

    Plasmid

    #115239

    Purpose

    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab5a (RAB5A Human, Homo sapiens)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Human UbC

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lck-FRB-ECFP(W66A)

    Plasmid

    #67902

    Purpose

    Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    Lck-FRB-ECFP(W66A)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046955

    Plasmid

    #145616

    Purpose

    Bacterial Expression plasmid for RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET-NEMO

    Plasmid

    #11966

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMO (IKBKG Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pNK869

    Plasmid

    #219737

    Purpose

    MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

    Insert

    promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
    

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    AtPD7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. T7RNAP Cyslight V174C

    Plasmid

    #205078

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V174C

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ki67∆repeats-mNeonGreen-IRESpuro2

    Plasmid

    #183742

    Purpose

    Expresses Ki67∆repeats-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67(lacking repeats) (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67(lacking repeats)

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p426 46Q GPD

    Plasmid

    #1182

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 46Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

18. PROM5_35Sx2

    Plasmid

    #136112

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35Sx2 (CaMV)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. PROM5_35S

    Plasmid

    #136111

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35S (CaMV)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pLK69

    Plasmid

    #37258

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    vang-like 2 (VANGL2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    lacks the 4 C-term aa

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only