We narrowed to 6,300 results for: nls

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pcDNA-Sin-Cas9

Plasmid

#227006

Purpose

Mammalian expression of Nuclease active S. iniae Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Spa-Cas9

Plasmid

#227007

Purpose

Mammalian expression of Nuclease active S. parasanguinis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sub-Cas9

Plasmid

#227008

Purpose

Mammalian expression of Nuclease active S. uberis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-FCPF

Plasmid

#220132

Purpose

Expresses Cas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

Cas9-FCPF (cas9 S. pyogenes)

Tags

His and NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-KRAB-2A-mTurquoise

Plasmid

#225690

Purpose

Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-ZNF10 KRAB

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GB3668

Plasmid

#215243

Purpose

TU for the expression of mAID (N-tag): AcrIIA4 w/o NLS protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and TNOS terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

P35S:mAID:AcrIIA4:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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GB1531

Plasmid

#215242

Purpose

TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

PNos:PhiC31:TNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKL2-138

Plasmid

#219413

Purpose

Estradiol-inducible expression of 42-10 Zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}

Expression

Yeast

Mutation

Four Arg to Ala mutations in zinc finger backbone

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ262F-ABE8e

Plasmid

#199179

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30

Insert

ABE8e(V106W)-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…

Tags

NLS

Expression

Plant

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-NlaCas7-p65

Plasmid

#207394

Purpose

Expresses NlaCas7-p65 activator fusion in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-NlaCas7-p65-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pENmRFPCNAL2 pc1054

Plasmid

#167567

Purpose

The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

Depositor

Cristina Cardoso

Article

Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ265N1

Plasmid

#173998

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E3E4 PUb-YFP

Plasmid

#173666

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
YFP

Tags

SV40 NLS

Expression

Insect

Mutation

Nix isoforms with exons E1, E3, E4

Promoter

Aedes aegypti PUb and own

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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EC2_2_dCas9_VP64_sgRNA

Plasmid

#163707

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VP64+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJT140_GalL_cCDA1-VRERBE3

Plasmid

#145103

Purpose

Expressing base editor cCDA1-VRERBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VRERBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT46_GalL_cCDA1-BE3

Plasmid

#145039

Purpose

Expresses cCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

cCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT176_GalL_eA3A-BE3

Plasmid

#145125

Purpose

Expressing base editor eA3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(N57G)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(N57G); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT171_GalL_A3AΔ178-BE3

Plasmid

#145121

Purpose

Expressing base editor A3AΔ178-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ178-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-178AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT170_GalL_A3AΔ182-BE3

Plasmid

#145120

Purpose

Expressing base editor A3AΔ182-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ182-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-182AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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