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pTAMAHISTEV_PfeAR480A

Plasmid

#128944

Purpose

expresses R480A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAG324V

Plasmid

#128947

Purpose

expresses G324V mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NOS-IN133.3xFLAG-WPRE

Plasmid

#127869

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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APBA3_PDZ_1

Plasmid

#103956

Purpose

Protein expression and purification of XIIL2 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

APBA3 (APBA3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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IL16_PDZ_3

Plasmid

#103929

Purpose

Protein expression and purification of IL16 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

IL16 (IL16 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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CYTIP_PDZ_1

Plasmid

#103930

Purpose

Protein expression and purification of PSCDBP PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

CYTIP (CYTIP Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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LIMK1_PDZ_1

Plasmid

#103932

Purpose

Protein expression and purification of LIMKINASE BGL1 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

LIMK1 (LIMK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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GRIP1_PDZ_4

Plasmid

#103934

Purpose

Protein expression and purification of GRIP PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

GRIP1 (GRIP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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APBA1_PDZ_1

Plasmid

#103942

Purpose

Protein expression and purification of APBA1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

APBA1 (APBA1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-C-SH2

Plasmid

#111419

Purpose

express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

Insert

murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-C-SH2+IA

Plasmid

#111418

Purpose

express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

Insert

murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-48-GFP

Plasmid

#108507

Purpose

expression of vsv-INCENP 1-48-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-58-GFP

Plasmid

#108508

Purpose

expression of vsv-INCENP 1-58-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-63-GFP

Plasmid

#108509

Purpose

expression of vsv-INCENP 1-63-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT1:HDT1-GFP

Plasmid

#108565

Purpose

Arabidopsis transformation, expresses HDT1/GFP fusion with HDT1 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT1 (HDA3 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

HDT1

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT1:GUS

Plasmid

#108441

Purpose

Arabidopsis transformation, expresses HDT1 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT1 promoter (HDA3 Mustard Weed)

Tags

GUS

Expression

Plant

Promoter

HDT1

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-565-YFP-566CT

Plasmid

#41677

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; YFP inserted d…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-570-CFP-571CT

Plasmid

#41680

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; CFP inserted d…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)mGAT1-570-YFP-571CT

Plasmid

#41679

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; YFP inserted d…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)mGAT1-565-CFP-566CT

Plasmid

#41678

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; CFP inserted d…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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